biostudies-literatureSponchiado MNHLBI NIH HHSNIH HHS1392443https://www.ebi.ac.uk/biostudies/studies/S-EPMC11070562biostudies-literatureUnknown15<b>Introduction:</b> Interleukin 13 (IL-13) is an important effector molecule in allergic asthma. IL-13-mediated mucin hypersecretion requires conversion of secretoglobin-positive club cells into goblet cells through suppression of <i>forkhead box A2</i> (<i>FOXA2</i>) and induction of <i>SAM pointed domain containing ETS transcription factor</i> (<i>SPDEF</i>). IL-13-mediated mucin hypersecretion may also include modulation of purinergic and muscarinic receptors that control basal and stimulated mucin secretion. We recently found that the transcription factor cAMP response element-binding protein (Creb1) inhibits <i>FOXA2</i> and modulates mucus secretion in mice. <b>Methods:</b> We tested the hypothesis that loss of club cell Creb1 mitigates the pro-mucin effects of IL-13. We challenged Frontiers in physiologySex-dependent regulation of mucin gene transcription and airway secretion and mechanics following intra-airway IL-13 in mice with conditional loss of club cell Creb1.PMC11070562R01 HL152101OT2 OD026582Trevizan-Bau PSponchiado MBonilla ALPrabhakaran SReznikov LRFagan AMata LfalseSex-dependent regulation of mucin gene transcription and airway secretion and mechanics following intra-airway IL-13 in mice with conditional loss of club cell Creb1.<b>Introduction:</b> Interleukin 13 (IL-13) is an important effector molecule in allergic asthma. IL-13-mediated mucin hypersecretion requires conversion of secretoglobin-positive club cells into goblet cells through suppression of <i>forkhead box A2</i> (<i>FOXA2</i>) and induction of <i>SAM pointed domain containing ETS transcription factor</i> (<i>SPDEF</i>). IL-13-mediated mucin hypersecretion may also include modulation of purinergic and muscarinic receptors that control basal and stimulated mucin secretion. We recently found that the transcription factor cAMP response element-binding protein (Creb1) inhibits <i>FOXA2</i> and modulates mucus secretion in mice. <b>Methods:</b> We tested the hypothesis that loss of club cell Creb1 mitigates the pro-mucin effects of IL-13. We challenged 2024-01-01T00:00:00Z20242026-06-01T08:04:07.639Z2026-04-08T10:48:26.911ZS-EPMC110705623871195110.3389/fphys.2024.1392443