{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chandler T"],"funding":["NIBIB NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the <i>three-dimensional orientations</i> and diffraction-limited positions of ensembles of fluorescent dipoles that label biological structures, and we share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model our samples, their excitation, and their detection using coarse-grained representations we call <i>orientation distribution functions</i> (ODFs). We apply ODFs to create physics-informed models of image formation with spatio-angular point-spread and transfer functions. We use theory and experiment to conclude that light-sheet tilting is a necessary part of our design for recovering all three-dimensional orientations. We use our system to extend known two-dimensional results to three dimensions in FM1-43-labelled giant unilamellar vesicles, fast-scarlet-labelled cellulose in xylem cells, and phalloidin-labelled actin in U2OS cells. Additionally, we observe phalloidin-labelled actin in mouse fibroblasts grown on grids of labelled nanowires and identify correlations between local actin alignment and global cell-scale orientation, indicating cellular coordination across length scales."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.03.09.584243"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11071302"],"repository":["biostudies-literature"],"pubmed_title":["Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM)."],"pmcid":["PMC11071302"],"funding_grant_id":["R35 GM131843","R01 EB026300"],"pubmed_authors":["Liu H","Liu J","Shroff H","Chen J","Mehta SB","Swaminathan V","Riviere P","Baskin TI","Fischer RS","Oldenbourg R","Agashe A","Chandler T","Guo M","Jamouille V","Nain A","Su Y","Kumar A","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM).","description":"Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the <i>three-dimensional orientations</i> and diffraction-limited positions of ensembles of fluorescent dipoles that label biological structures, and we share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model our samples, their excitation, and their detection using coarse-grained representations we call <i>orientation distribution functions</i> (ODFs). We apply ODFs to create physics-informed models of image formation with spatio-angular point-spread and transfer functions. We use theory and experiment to conclude that light-sheet tilting is a necessary part of our design for recovering all three-dimensional orientations. We use our system to extend known two-dimensional results to three dimensions in FM1-43-labelled giant unilamellar vesicles, fast-scarlet-labelled cellulose in xylem cells, and phalloidin-labelled actin in U2OS cells. Additionally, we observe phalloidin-labelled actin in mouse fibroblasts grown on grids of labelled nanowires and identify correlations between local actin alignment and global cell-scale orientation, indicating cellular coordination across length scales.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-26T03:18:20.815Z","creation":"2026-06-26T03:09:28.97Z"},"accession":"S-EPMC11071302","cross_references":{"pubmed":["38712306"],"doi":["10.1101/2024.03.09.584243"]}}