{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chang L"],"funding":["NHGRI NIH HHS","NCI NIH HHS","NINDS NIH HHS","NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["Comprehensive analysis of chromatin architecture is crucial for understanding the gene regulatory programs during development and in disease pathogenesis, yet current methods often inadequately address the unique challenges presented by analysis of heterogeneous tissue samples. Here, we introduce Droplet Hi-C, which employs a commercial microfluidic device for high-throughput, single-cell chromatin conformation profiling in droplets. Using Droplet Hi-C, we mapped the chromatin architecture at single-cell resolution from the mouse cortex and analyzed gene regulatory programs in major cortical cell types. Additionally, we used this technique to detect copy number variation (CNV), structural variations (SVs) and extrachromosomal DNA (ecDNA) in cancer cells, revealing clonal dynamics and other"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.04.18.590148"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11071305"],"repository":["biostudies-literature"],"pubmed_title":["Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single Cells."],"pmcid":["PMC11071305"],"funding_grant_id":["T32 GM007198","S10 OD026929","R56 NS080939","UM1 HG011585","R01 CA258248","R35 HG011922","T32 CA067754"],"pubmed_authors":["Chang L","Bejar R","Xie Y","Furnari FB","Hu M","Chen CC","Taylor B","Wang Z","Tan TR","Sun J","Ren B"],"additional_accession":[]},"is_claimable":false,"name":"Droplet Hi-C for Fast and Scalable Profiling of Chromatin Architecture in Single Cells.","description":"Comprehensive analysis of chromatin architecture is crucial for understanding the gene regulatory programs during development and in disease pathogenesis, yet current methods often inadequately address the unique challenges presented by analysis of heterogeneous tissue samples. Here, we introduce Droplet Hi-C, which employs a commercial microfluidic device for high-throughput, single-cell chromatin conformation profiling in droplets. Using Droplet Hi-C, we mapped the chromatin architecture at single-cell resolution from the mouse cortex and analyzed gene regulatory programs in major cortical cell types. Additionally, we used this technique to detect copy number variation (CNV), structural variations (SVs) and extrachromosomal DNA (ecDNA) in cancer cells, revealing clonal dynamics and other","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-07-02T03:13:20.139Z","creation":"2026-07-02T03:09:01.363Z"},"accession":"S-EPMC11071305","cross_references":{"pubmed":["38712075"],"doi":["10.1101/2024.04.18.590148"]}}