biostudies-literatureLi HNational Key R&D Program of ChinaNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaFok Ying Tung Education Foundation467https://www.ebi.ac.uk/biostudies/studies/S-EPMC11071816biostudies-literatureUnknown79(8)Meiosis, a highly conserved process in organisms from fungi to mammals, is subjected to protein phosphorylation regulation. Due to the low abundance of phosphorylation, there is a lack of systemic characterization of phosphorylation regulation of meiosis in mammals. Using the phosphoproteomic approach, we profiled large-scale phosphoproteome of purified primary spermatocytes undergoing meiosis I, and identified 14,660 phosphorylation sites in 4419 phosphoproteins. Kinase-substrate phosphorylation network analysis followed by in vitro meiosis study showed that CDK9 was essential for meiosis progression to metaphase I and had enriched substrate phosphorylation sites in proteins involved in meiotic cell cycle. In addition, histones and epigenetic factors were found to be widely phosphorylatedCellular and molecular life sciences : CMLSGlobal phosphoproteomic analysis identified key kinases regulating male meiosis in mouse.PMC110718162021YFC2700200820016118197143916103732071133Yao LLi HWang BGuo YGuo XZhu HChen HZhao YLi YCui YRen JZhang XQi YChen YSitu CWang YZhu TfalseGlobal phosphoproteomic analysis identified key kinases regulating male meiosis in mouse.Meiosis, a highly conserved process in organisms from fungi to mammals, is subjected to protein phosphorylation regulation. Due to the low abundance of phosphorylation, there is a lack of systemic characterization of phosphorylation regulation of meiosis in mammals. Using the phosphoproteomic approach, we profiled large-scale phosphoproteome of purified primary spermatocytes undergoing meiosis I, and identified 14,660 phosphorylation sites in 4419 phosphoproteins. Kinase-substrate phosphorylation network analysis followed by in vitro meiosis study showed that CDK9 was essential for meiosis progression to metaphase I and had enriched substrate phosphorylation sites in proteins involved in meiotic cell cycle. In addition, histones and epigenetic factors were found to be widely phosphorylated2022-01-01T00:00:00Z2022 Aug2026-06-01T08:06:14.251Z2026-04-08T10:51:13.586ZS-EPMC110718163593008010.1007/s00018-022-04507-8