{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chang CC"],"funding":["Ministry of Science and Technology of the People's Republic of China","Ministry of Science and Technology of the People's Republic of China","Medical Research Council","National Natural Science Foundation of China","Shanghai Science and Technology Commission"],"pagination":["420"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11072341"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["79(8)"],"pubmed_abstract":["The cytoophidium is a unique type of membraneless compartment comprising of filamentous protein polymers. Inosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step of de novo GTP biosynthesis and plays critical roles in active cell metabolism. However, the molecular regulation of cytoophidium formation is poorly understood. Here we show that human IMPDH2 polymers bundle up to form cytoophidium-like aggregates in vitro when macromolecular crowders are present. The self-association of IMPDH polymers is suggested to rely on electrostatic interactions. In cells, the increase of molecular crowding with hyperosmotic medium induces cytoophidia, while the decrease of that by the inhibition of RNA synthesis perturbs cytoophidium assembly. In addition to IMPDH, CTPS and PRPS cytoo"],"journal":["Cellular and molecular life sciences : CMLS"],"pubmed_title":["Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation."],"pmcid":["PMC11072341"],"funding_grant_id":["20JC1410500","MC_UU_12021/3","31771490","MC_U137788471","2021YFA0804701-4"],"pubmed_authors":["Chang CC","Zhang Z","Keppeke GD","Liu JL","Sung LY","Zhong J","Peng M"],"additional_accession":[]},"is_claimable":false,"name":"Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation.","description":"The cytoophidium is a unique type of membraneless compartment comprising of filamentous protein polymers. Inosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step of de novo GTP biosynthesis and plays critical roles in active cell metabolism. However, the molecular regulation of cytoophidium formation is poorly understood. Here we show that human IMPDH2 polymers bundle up to form cytoophidium-like aggregates in vitro when macromolecular crowders are present. The self-association of IMPDH polymers is suggested to rely on electrostatic interactions. In cells, the increase of molecular crowding with hyperosmotic medium induces cytoophidia, while the decrease of that by the inhibition of RNA synthesis perturbs cytoophidium assembly. In addition to IMPDH, CTPS and PRPS cytoo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-05-27T03:19:40.557Z","creation":"2026-05-27T03:11:59.646Z"},"accession":"S-EPMC11072341","cross_references":{"pubmed":["35833994"],"doi":["10.1007/s00018-022-04448-2"]}}