{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["78(1)"],"submitter":["Zhang M"],"pubmed_abstract":["Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (spMis18, spMis16 and spMis19) is located to the centromere to license the recruitment of Cnp1<sup>CENP-A</sup> in Schizosaccharomyces pombe. We found that spMis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A-H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of t"],"journal":["Cellular and molecular life sciences : CMLS"],"pagination":["373-384"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11073290"],"repository":["biostudies-literature"],"pubmed_title":["Centromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast."],"pmcid":["PMC11073290"],"pubmed_authors":["Xiong Y","Wu M","Dong F","Fu C","Zang J","Wang C","Zhang X","Zhang M","Zheng F","Niu X","Shao C"],"additional_accession":[]},"is_claimable":false,"name":"Centromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast.","description":"Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (spMis18, spMis16 and spMis19) is located to the centromere to license the recruitment of Cnp1<sup>CENP-A</sup> in Schizosaccharomyces pombe. We found that spMis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A-H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of t","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2026-06-02T10:27:09.652Z","creation":"2026-05-26T03:07:32.584Z"},"accession":"S-EPMC11073290","cross_references":{"pubmed":["32318758"],"doi":["10.1007/s00018-020-03502-1"]}}