biostudies-literatureUnknown78(1)Zhang MFaithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (spMis18, spMis16 and spMis19) is located to the centromere to license the recruitment of Cnp1^CENP-A in Schizosaccharomyces pombe. We found that spMis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A-H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of tCellular and molecular life sciences : CMLS373-384https://www.ebi.ac.uk/biostudies/studies/S-EPMC11073290biostudies-literatureCentromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast.PMC11073290Xiong YWu MDong FFu CZang JWang CZhang XZhang MZheng FNiu XShao CfalseCentromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast.Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (spMis18, spMis16 and spMis19) is located to the centromere to license the recruitment of Cnp1^CENP-A in Schizosaccharomyces pombe. We found that spMis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A-H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of t2021-01-01T00:00:00Z2021 Jan2026-06-02T10:27:09.652Z2026-05-26T03:07:32.584ZS-EPMC110732903231875810.1007/s00018-020-03502-1