biostudies-literatureUnknown4Ayayee PA<h4>Introduction</h4>Building on our previous work, we investigate how dietary shifts affect gut microbial essential amino acid (EAA) provisioning in the lactating cockroach <i>Diploptera punctata</i>.<h4>Method</h4>To that end, we fed cockroaches three distinct diets: a plant-only Gari diet composed of starchy and granulated root tuber Yucca (<i>Manihot esculenta</i>), a dog food diet (DF), and a cellulose-amended dog food (CADF) diet. We anticipated that the high carbohydrate, low protein Gari would highlight increased microbial EAA supplementation to the host.<h4>Results</h4>By day 28, we observed distinct profiles of 14 bacterial families in the insect gut microbiomes of the three dietary groups. CADF-fed insects predominantly harbored cellulolytic and nitrogen-fixing bacteria familiesFrontiers in insect science1396984https://www.ebi.ac.uk/biostudies/studies/S-EPMC11073486biostudies-literatureEnhanced gut microbiome supplementation of essential amino acids in <i>Diploptera punctata</i> fed low-protein plant-based diet.PMC11073486Larsen TAyayee PAPetersen NRauter CRiusch JfalseEnhanced gut microbiome supplementation of essential amino acids in <i>Diploptera punctata</i> fed low-protein plant-based diet.<h4>Introduction</h4>Building on our previous work, we investigate how dietary shifts affect gut microbial essential amino acid (EAA) provisioning in the lactating cockroach <i>Diploptera punctata</i>.<h4>Method</h4>To that end, we fed cockroaches three distinct diets: a plant-only Gari diet composed of starchy and granulated root tuber Yucca (<i>Manihot esculenta</i>), a dog food diet (DF), and a cellulose-amended dog food (CADF) diet. We anticipated that the high carbohydrate, low protein Gari would highlight increased microbial EAA supplementation to the host.<h4>Results</h4>By day 28, we observed distinct profiles of 14 bacterial families in the insect gut microbiomes of the three dietary groups. CADF-fed insects predominantly harbored cellulolytic and nitrogen-fixing bacteria families2024-01-01T00:00:00Z20242026-06-01T17:26:57.861Z2026-04-08T14:14:30.674ZS-EPMC110734863871146310.3389/finsc.2024.1396984