biostudies-literatureMartin-Roy RCarlsbergfondetAgence Nationale de la RechercheDanmarks Frie ForskningsfondCentre National de la Recherche Scientifiquee0302646https://www.ebi.ac.uk/biostudies/studies/S-EPMC11073703biostudies-literatureUnknown19(5)The analysis of the DNA entrapped in ancient shells of molluscs has the potential to shed light on the evolution and ecology of this very diverse phylum. Ancient genomics could help reconstruct the responses of molluscs to past climate change, pollution, and human subsistence practices at unprecedented temporal resolutions. Applications are however still in their infancy, partly due to our limited knowledge of DNA preservation in calcium carbonate shells and the need for optimized methods for responsible genomic data generation. To improve ancient shell genomic analyses, we applied high-throughput DNA sequencing to 27 Mytilus mussel shells dated to ~111-6500 years Before Present, and investigated the impact, on DNA recovery, of shell imaging, DNA extraction protocols and shell sub-samplingPloS oneAdvancing responsible genomic analyses of ancient mollusc shells.PMC11073703Tellus-INTERRVIE-MAELSTROMMITI-Adaptation2020-X-Shell7027-00060BANR-18-CE02-0014-02ANR-20-CE02-0024CF21-0564Gotfredsen ABHansen CHBangsgaard PChristiansen GMartin-Roy RMata XIlsoe PCFunder SMarin FBennike ODer Sarkissian CThyrring JGregersen KMKlassen LKristensen IKRavnholt GBfalseAdvancing responsible genomic analyses of ancient mollusc shells.The analysis of the DNA entrapped in ancient shells of molluscs has the potential to shed light on the evolution and ecology of this very diverse phylum. Ancient genomics could help reconstruct the responses of molluscs to past climate change, pollution, and human subsistence practices at unprecedented temporal resolutions. Applications are however still in their infancy, partly due to our limited knowledge of DNA preservation in calcium carbonate shells and the need for optimized methods for responsible genomic data generation. To improve ancient shell genomic analyses, we applied high-throughput DNA sequencing to 27 Mytilus mussel shells dated to ~111-6500 years Before Present, and investigated the impact, on DNA recovery, of shell imaging, DNA extraction protocols and shell sub-sampling2024-01-01T00:00:00Z20242026-05-27T03:16:02.007Z2026-05-27T03:08:12.913ZS-EPMC110737033870976610.1371/journal.pone.0302646