biostudies-literatureXu LThis research was supported by the Science and Technology Development Project of Jilin Province180https://www.ebi.ac.uk/biostudies/studies/S-EPMC11075196biostudies-literatureUnknown20(1)<h4>Background</h4>Infectious bovine rhinotracheitis (IBR), caused by Bovine alphaherpesvirus-1 (BoAHV-1), is an acute, highly contagious disease primarily characterized by respiratory tract lesions in infected cattle. Due to its severe pathological damage and extensive transmission, it results in significant economic losses in the cattle industry. Accurate detection of BoAHV-1 is of paramount importance. In this study, we developed a real-time fluorescent quantitative PCR detection method for detecting BoAHV-1 infections. Utilizing this method, we tested clinical samples and successfully identified and isolated a strain of BoAHV-1.1 from positive samples. Subsequently, we conducted a genetic evolution analysis on the isolate strain's gC, TK, gG, gD, and gE genes.<h4>Results</h4>The study BMC veterinary researchEstablishment of a real-time fluorescent quantitative PCR detection method and phylogenetic analysis of BoAHV-1.PMC1107519620220101332JCXu LLiu YLi JDu RGe GLi DGong QCui ZLeng XShi KLiu FDiao NfalseEstablishment of a real-time fluorescent quantitative PCR detection method and phylogenetic analysis of BoAHV-1.<h4>Background</h4>Infectious bovine rhinotracheitis (IBR), caused by Bovine alphaherpesvirus-1 (BoAHV-1), is an acute, highly contagious disease primarily characterized by respiratory tract lesions in infected cattle. Due to its severe pathological damage and extensive transmission, it results in significant economic losses in the cattle industry. Accurate detection of BoAHV-1 is of paramount importance. In this study, we developed a real-time fluorescent quantitative PCR detection method for detecting BoAHV-1 infections. Utilizing this method, we tested clinical samples and successfully identified and isolated a strain of BoAHV-1.1 from positive samples. Subsequently, we conducted a genetic evolution analysis on the isolate strain's gC, TK, gG, gD, and gE genes.<h4>Results</h4>The study 2024-01-01T00:00:00Z2024 May2026-06-01T08:04:28.409Z2026-04-08T10:47:07.112ZS-EPMC110751963871502810.1186/s12917-024-04025-8