biostudies-literatureUnknown14(4)Cui WGenetic and epigenetic aberrations display an essential role in the initiation and progression of diffuse large B-cell lymphoma (DLBCL). 5-methylcytosine (m⁵C), a common RNA modification, regulates various cellular processes and contributes to tumorigenesis and cancer progression. However, m⁵C alterations in DLBCL remain unclear. Our research constructed an m⁵C prognostic model utilizing GEO data sets, which can efficiently predict the prognosis of patients with DLBCL, and verified the m⁵C prognostic model genes by immunohistochemistry analysis. This model was constructed using unsupervised consensus clustering analyses, Least Absolute Shrinkage and Selection Operator (LASSO), and multivariate Cox regression analyses. Based on the expression of mAmerican journal of cancer research1768-1783https://www.ebi.ac.uk/biostudies/studies/S-EPMC11076244biostudies-literatureRoles of RNA m⁵C modification patterns in prognosis and tumor microenvironment infiltration of diffuse large B-cell lymphoma.PMC11076244Zhou LYu LYu QCui WTang DLuo CMeng YLuo QLei ZSun RYu TWang YPeng LfalseRoles of RNA m⁵C modification patterns in prognosis and tumor microenvironment infiltration of diffuse large B-cell lymphoma.Genetic and epigenetic aberrations display an essential role in the initiation and progression of diffuse large B-cell lymphoma (DLBCL). 5-methylcytosine (m⁵C), a common RNA modification, regulates various cellular processes and contributes to tumorigenesis and cancer progression. However, m⁵C alterations in DLBCL remain unclear. Our research constructed an m⁵C prognostic model utilizing GEO data sets, which can efficiently predict the prognosis of patients with DLBCL, and verified the m⁵C prognostic model genes by immunohistochemistry analysis. This model was constructed using unsupervised consensus clustering analyses, Least Absolute Shrinkage and Selection Operator (LASSO), and multivariate Cox regression analyses. Based on the expression of m2024-01-01T00:00:00Z20242026-06-02T02:20:57.682Z2025-08-14T03:05:48.181ZS-EPMC110762443872628510.62347/NXDR182610.62347/nxdr1826