{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(5)"],"submitter":["Anchundia DJ"],"pubmed_abstract":["Incredibly powerful whole genome studies of conservation genetics, evolution, and biogeography become possible for non-model organisms when reference genomes are available. Here, we report the sequence and assembly of the whole genome of the little vermilion flycatcher (Pyrocephalus nanus; family Tyrannidae), which is an endemic, endangered, and declining species of the Galapagos Islands. Using PacBio HiFi reads to assemble long contigs and Hi-C reads for scaffolding, we assembled a genome of 1.07 Gb comprising 267 contigs in 152 scaffolds, scaffold N50 74 M, contig N50 17.8 M, with 98.9% assigned to candidate chromosomal sequences and 99.72% of the BUSCO passeriformes 10,844 single-copy orthologs present. In addition, we used the novel HiFiMiTie pipeline to fully assemble and verify all p"],"journal":["Genome biology and evolution"],"pagination":["evae083"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11077314"],"repository":["biostudies-literature"],"pubmed_title":["Genome Assembly of Pyrocephalus nanus: A Step Toward the Genetic Conservation of the Endangered Little Vermilion Flycatcher of the Galapagos Islands."],"pmcid":["PMC11077314"],"pubmed_authors":["Henderson JB","Anchundia DJ","Lam AW","Dumbacher JP","Van Dam MH"],"additional_accession":[]},"is_claimable":false,"name":"Genome Assembly of Pyrocephalus nanus: A Step Toward the Genetic Conservation of the Endangered Little Vermilion Flycatcher of the Galapagos Islands.","description":"Incredibly powerful whole genome studies of conservation genetics, evolution, and biogeography become possible for non-model organisms when reference genomes are available. Here, we report the sequence and assembly of the whole genome of the little vermilion flycatcher (Pyrocephalus nanus; family Tyrannidae), which is an endemic, endangered, and declining species of the Galapagos Islands. Using PacBio HiFi reads to assemble long contigs and Hi-C reads for scaffolding, we assembled a genome of 1.07 Gb comprising 267 contigs in 152 scaffolds, scaffold N50 74 M, contig N50 17.8 M, with 98.9% assigned to candidate chromosomal sequences and 99.72% of the BUSCO passeriformes 10,844 single-copy orthologs present. In addition, we used the novel HiFiMiTie pipeline to fully assemble and verify all p","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-05-27T03:09:22.401Z","creation":"2025-08-14T03:05:38.196Z"},"accession":"S-EPMC11077314","cross_references":{"pubmed":["38652799"],"doi":["10.1093/gbe/evae083"]}}