{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Houston S"],"funding":["Natural Sciences and Engineering Research Council of Canada","Social Sciences and Humanities Research Council of Canada","NIAID NIH HHS","Open Philanthropy Project","Division of Intramural Research","Canadian Institutes of Health Research"],"pagination":["1725-1743"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11077495"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(5)"],"pubmed_abstract":["Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all <i>Treponema pallidum</i> proteins under infection conditions (in vivo-grown <i>T. pallidum</i>). Recently, a method was developed for the long-term culture of <i>T. pallidum</i> under in vitro conditions (in vitro-cultured <i>T. pallidum</i>). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the <i>T. pallidum</i> global protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined <i>T. pallidum</i> proteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire of"],"journal":["Journal of proteome research"],"pubmed_title":["In-Depth Proteome Coverage of In Vitro-Cultured <i>Treponema pallidum</i> and Quantitative Comparison Analyses with In Vivo-Grown Treponemes."],"pmcid":["PMC11077495"],"funding_grant_id":["R37AI051334","R37 AI051334","52345","U19AI144133","U19 AI144133","U01 AI182035"],"pubmed_authors":["Goodyear MC","Geppert A","Cameron CE","Houston S","Gomez A"],"additional_accession":[]},"is_claimable":false,"name":"In-Depth Proteome Coverage of In Vitro-Cultured <i>Treponema pallidum</i> and Quantitative Comparison Analyses with In Vivo-Grown Treponemes.","description":"Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all <i>Treponema pallidum</i> proteins under infection conditions (in vivo-grown <i>T. pallidum</i>). Recently, a method was developed for the long-term culture of <i>T. pallidum</i> under in vitro conditions (in vitro-cultured <i>T. pallidum</i>). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the <i>T. pallidum</i> global protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined <i>T. pallidum</i> proteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire of","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-03T06:50:51.698Z","creation":"2026-04-25T03:22:05.099Z"},"accession":"S-EPMC11077495","cross_references":{"pubmed":["38636938"],"doi":["10.1021/acs.jproteome.3c00891"]}}