biostudies-literatureHouston SNatural Sciences and Engineering Research Council of CanadaSocial Sciences and Humanities Research Council of CanadaNIAID NIH HHSOpen Philanthropy ProjectDivision of Intramural ResearchCanadian Institutes of Health Research1725-1743https://www.ebi.ac.uk/biostudies/studies/S-EPMC11077495biostudies-literatureUnknown23(5)Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all <i>Treponema pallidum</i> proteins under infection conditions (in vivo-grown <i>T. pallidum</i>). Recently, a method was developed for the long-term culture of <i>T. pallidum</i> under in vitro conditions (in vitro-cultured <i>T. pallidum</i>). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the <i>T. pallidum</i> global protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined <i>T. pallidum</i> proteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire ofJournal of proteome researchIn-Depth Proteome Coverage of In Vitro-Cultured <i>Treponema pallidum</i> and Quantitative Comparison Analyses with In Vivo-Grown Treponemes.PMC11077495R37AI051334R37 AI05133452345U19AI144133U19 AI144133U01 AI182035Goodyear MCGeppert ACameron CEHouston SGomez AfalseIn-Depth Proteome Coverage of In Vitro-Cultured <i>Treponema pallidum</i> and Quantitative Comparison Analyses with In Vivo-Grown Treponemes.Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all <i>Treponema pallidum</i> proteins under infection conditions (in vivo-grown <i>T. pallidum</i>). Recently, a method was developed for the long-term culture of <i>T. pallidum</i> under in vitro conditions (in vitro-cultured <i>T. pallidum</i>). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the <i>T. pallidum</i> global protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined <i>T. pallidum</i> proteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire of2024-01-01T00:00:00Z2024 May2026-06-03T06:50:51.698Z2026-04-25T03:22:05.099ZS-EPMC110774953863693810.1021/acs.jproteome.3c00891