{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Steinmetz TD"],"funding":["Universit?t zu K?ln","Deutsche Forschungsgemeinschaft"],"pagination":["1615-1633"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11077586"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(5)"],"pubmed_abstract":["Autophagy supervises the proteostasis and survival of B lymphocytic cells. <i>Trk-fused gene</i> (TFG) promotes autophagosome-lysosome flux in murine CH12 B cells, as well as their survival. Hence, quantitative proteomics of CH12<i>tfg</i>KO and WT B cells in combination with lysosomal inhibition should identify proteins that are prone to lysosomal degradation and contribute to autophagy and B cell survival. Lysosome inhibition via NH<sub>4</sub>Cl unexpectedly reduced a number of proteins but increased a large cluster of translational, ribosomal, and mitochondrial proteins, independent of TFG. Hence, we propose a role for lysosomes in ribophagy in B cells. TFG-regulated proteins include CD74, BCL10, or the immunoglobulin JCHAIN. Gene ontology (GO) analysis reveals that proteins regulated "],"journal":["Journal of proteome research"],"pubmed_title":["Identification of TFG- and Autophagy-Regulated Proteins and Glycerophospholipids in B Cells."],"pmcid":["PMC11077586"],"funding_grant_id":["EXC-2189  390939984","GK1660","939/6-1","TRR130","403222702 - SFB 1381"],"pubmed_authors":["Castiglione K","Jack HM","Himmelreich AK","Brodesser S","Mielenz D","Steinmetz TD","Schulz SR","Thomas J","Warscheid B","Reimann L","Golombek F"],"additional_accession":[]},"is_claimable":false,"name":"Identification of TFG- and Autophagy-Regulated Proteins and Glycerophospholipids in B Cells.","description":"Autophagy supervises the proteostasis and survival of B lymphocytic cells. <i>Trk-fused gene</i> (TFG) promotes autophagosome-lysosome flux in murine CH12 B cells, as well as their survival. Hence, quantitative proteomics of CH12<i>tfg</i>KO and WT B cells in combination with lysosomal inhibition should identify proteins that are prone to lysosomal degradation and contribute to autophagy and B cell survival. Lysosome inhibition via NH<sub>4</sub>Cl unexpectedly reduced a number of proteins but increased a large cluster of translational, ribosomal, and mitochondrial proteins, independent of TFG. Hence, we propose a role for lysosomes in ribophagy in B cells. TFG-regulated proteins include CD74, BCL10, or the immunoglobulin JCHAIN. Gene ontology (GO) analysis reveals that proteins regulated ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-02T06:54:37.736Z","creation":"2026-04-15T03:15:35.033Z"},"accession":"S-EPMC11077586","cross_references":{"pubmed":["38649144"],"doi":["10.1021/acs.jproteome.3c00713"]}}