biostudies-literatureSteinmetz TDUniversit?t zu K?lnDeutsche Forschungsgemeinschaft1615-1633https://www.ebi.ac.uk/biostudies/studies/S-EPMC11077586biostudies-literatureUnknown23(5)Autophagy supervises the proteostasis and survival of B lymphocytic cells. <i>Trk-fused gene</i> (TFG) promotes autophagosome-lysosome flux in murine CH12 B cells, as well as their survival. Hence, quantitative proteomics of CH12<i>tfg</i>KO and WT B cells in combination with lysosomal inhibition should identify proteins that are prone to lysosomal degradation and contribute to autophagy and B cell survival. Lysosome inhibition via NH₄Cl unexpectedly reduced a number of proteins but increased a large cluster of translational, ribosomal, and mitochondrial proteins, independent of TFG. Hence, we propose a role for lysosomes in ribophagy in B cells. TFG-regulated proteins include CD74, BCL10, or the immunoglobulin JCHAIN. Gene ontology (GO) analysis reveals that proteins regulated Journal of proteome researchIdentification of TFG- and Autophagy-Regulated Proteins and Glycerophospholipids in B Cells.PMC11077586EXC-2189 390939984GK1660939/6-1TRR130403222702 - SFB 1381Castiglione KJack HMHimmelreich AKBrodesser SMielenz DSteinmetz TDSchulz SRThomas JWarscheid BReimann LGolombek FfalseIdentification of TFG- and Autophagy-Regulated Proteins and Glycerophospholipids in B Cells.Autophagy supervises the proteostasis and survival of B lymphocytic cells. <i>Trk-fused gene</i> (TFG) promotes autophagosome-lysosome flux in murine CH12 B cells, as well as their survival. Hence, quantitative proteomics of CH12<i>tfg</i>KO and WT B cells in combination with lysosomal inhibition should identify proteins that are prone to lysosomal degradation and contribute to autophagy and B cell survival. Lysosome inhibition via NH₄Cl unexpectedly reduced a number of proteins but increased a large cluster of translational, ribosomal, and mitochondrial proteins, independent of TFG. Hence, we propose a role for lysosomes in ribophagy in B cells. TFG-regulated proteins include CD74, BCL10, or the immunoglobulin JCHAIN. Gene ontology (GO) analysis reveals that proteins regulated 2024-01-01T00:00:00Z2024 May2026-06-02T06:54:37.736Z2026-04-15T03:15:35.033ZS-EPMC110775863864914410.1021/acs.jproteome.3c00713