biostudies-literatureKim YSNational Research Foundation of Korea (N.R.F.) grantChonnam National University Hospital Biomedical Research Institute26https://www.ebi.ac.uk/biostudies/studies/S-EPMC11077708biostudies-literatureUnknown16(1)<h4>Background/aims</h4>Bile reflux (BR) can influence the gastric environment by altering gastric acidity and possibly the gastric microbiota composition. This study investigated the correlation between bile acids and microbial compositions in the gastric juice of 50 subjects with differing gastric pathologies.<h4>Methods</h4>This study included 50 subjects, which were categorized into three groups based on the endoscopic BR grading system. The primary and secondary bile acid concentrations in gastric juice samples were measured, and microbiota profiling was conducted using 16 S rRNA gene sequencing.<h4>Results</h4>Significant differences were observed in each bile acid level in the three endoscopic BR groups (P < 0.05). The Shannon index demonstrated a significant decrease in the higher Gut pathogensEffect of bile reflux on gastric juice microbiota in patients with different histology phenotypes.PMC11077708BCRI 23033grant number: 2021R1F1A105950Lee SMKim DHUnno TJung YDChung JOChoi YDKim HSCho SHPark SYKim YSfalseEffect of bile reflux on gastric juice microbiota in patients with different histology phenotypes.<h4>Background/aims</h4>Bile reflux (BR) can influence the gastric environment by altering gastric acidity and possibly the gastric microbiota composition. This study investigated the correlation between bile acids and microbial compositions in the gastric juice of 50 subjects with differing gastric pathologies.<h4>Methods</h4>This study included 50 subjects, which were categorized into three groups based on the endoscopic BR grading system. The primary and secondary bile acid concentrations in gastric juice samples were measured, and microbiota profiling was conducted using 16 S rRNA gene sequencing.<h4>Results</h4>Significant differences were observed in each bile acid level in the three endoscopic BR groups (P < 0.05). The Shannon index demonstrated a significant decrease in the higher 2024-01-01T00:00:00Z2024 May2026-03-12T23:05:59.069Z2025-08-12T03:04:55.532ZS-EPMC110777083871510110.1186/s13099-024-00619-7