biostudies-literatureGurung PNIAID NIH HHSHHS | NIH | National Institute of Allergy and Infectious Diseasese0285023https://www.ebi.ac.uk/biostudies/studies/S-EPMC11078010biostudies-literatureUnknown15(5)Condensin I is a pentameric complex that regulates the mitotic chromosome assembly in eukaryotes. The kleisin subunit CAP-H of the condensin I complex acts as a linchpin to maintain the structural integrity and loading of this complex on mitotic chromosomes. This complex is present in all eukaryotes and has recently been identified in <i>Plasmodium</i> spp. However, how this complex is assembled and whether the kleisin subunit is critical for this complex in these parasites are yet to be explored. To examine the role of PfCAP-H during cell division within erythrocytes, we generated an inducible PfCAP-H knockout parasite. We find that PfCAP-H is dynamically expressed during mitosis with the peak expression at the metaphase plate. PfCAP-H interacts with PfCAP-G and is a non-SMC member of themBioPfCAP-H is essential for assembly of condensin I complex and karyokinesis during asexual proliferation of <i>Plasmodium falciparum</i>.PMC11078010R01 AI145941R01AI145941Gurung PMcGee JPDvorin JDfalsePfCAP-H is essential for assembly of condensin I complex and karyokinesis during asexual proliferation of <i>Plasmodium falciparum</i>.Condensin I is a pentameric complex that regulates the mitotic chromosome assembly in eukaryotes. The kleisin subunit CAP-H of the condensin I complex acts as a linchpin to maintain the structural integrity and loading of this complex on mitotic chromosomes. This complex is present in all eukaryotes and has recently been identified in <i>Plasmodium</i> spp. However, how this complex is assembled and whether the kleisin subunit is critical for this complex in these parasites are yet to be explored. To examine the role of PfCAP-H during cell division within erythrocytes, we generated an inducible PfCAP-H knockout parasite. We find that PfCAP-H is dynamically expressed during mitosis with the peak expression at the metaphase plate. PfCAP-H interacts with PfCAP-G and is a non-SMC member of the2024-01-01T00:00:00Z2024 May2026-06-02T06:54:25.527Z2026-04-15T03:15:33.049ZS-EPMC110780103856467610.1128/mbio.02850-23