biostudies-literatureSivalogan KNational Institute of Environmental Health SciencesNIEHS NIH HHSNational Institutes of HealthBill and Melinda Gates Foundation102144https://www.ebi.ac.uk/biostudies/studies/S-EPMC11079463biostudies-literatureUnknown8(5)<h4>Background</h4>Maternal overweight and obesity has been associated with poor lactation performance including delayed lactogenesis and reduced duration. However, the effect on human milk composition is less well understood.<h4>Objectives</h4>We evaluated the relationship of maternal BMI on the human milk metabolome among Guatemalan mothers.<h4>Methods</h4>We used data from 75 Guatemalan mothers who participated in the Household Air Pollution Intervention Network trial. Maternal BMI was measured between 9 and <20 weeks of gestation. Milk samples were collected at a single time point using aseptic collection from one breast at 6 mo postpartum and analyzed using high-resolution mass spectrometry. A cross-sectional untargeted high-resolution metabolomics analysis was performed by coupling hCurrent developments in nutritionHuman Milk Composition Is Associated with Maternal Body Mass Index in a Cross-Sectional, Untargeted Metabolomics Analysis of Human Milk from Guatemalan Mothers.PMC11079463T32 ES0128701UM1HL134590OPP1131279P30 ES019776Liang DAccardi CDiaz-Artiga AHu XTran VSinharoy SSTeeny SNSivalogan KClasen TFRamakrishnan UMollinedo EThompson LMfalseHuman Milk Composition Is Associated with Maternal Body Mass Index in a Cross-Sectional, Untargeted Metabolomics Analysis of Human Milk from Guatemalan Mothers.<h4>Background</h4>Maternal overweight and obesity has been associated with poor lactation performance including delayed lactogenesis and reduced duration. However, the effect on human milk composition is less well understood.<h4>Objectives</h4>We evaluated the relationship of maternal BMI on the human milk metabolome among Guatemalan mothers.<h4>Methods</h4>We used data from 75 Guatemalan mothers who participated in the Household Air Pollution Intervention Network trial. Maternal BMI was measured between 9 and <20 weeks of gestation. Milk samples were collected at a single time point using aseptic collection from one breast at 6 mo postpartum and analyzed using high-resolution mass spectrometry. A cross-sectional untargeted high-resolution metabolomics analysis was performed by coupling h2024-01-01T00:00:00Z2024 May2026-06-02T01:37:04.631Z2026-04-13T03:12:17.412ZS-EPMC110794633872602710.1016/j.cdnut.2024.102144