biostudies-literatureZhang CPeking University Medicine Sailing Program for Young Scholars’ Scientific & Technological InnovationPeking University Medicine Sailing Program for Young Scholars' Scientific & Technological InnovationNatural Science Foundation of ChinaBeijing Nova ProgramTechnology special fund of Hainan Province123https://www.ebi.ac.uk/biostudies/studies/S-EPMC11087383biostudies-literatureUnknown73(7)Adoptively transferred T cell receptor-engineered T cells are a promising cancer treatment strategy, and the identification of tumour-specific TCRs is essential. Previous studies reported that tumour-reactive T cells and TCRs could be isolated based on the expression of activation markers. However, since T cells with different cell states could not respond uniformly to activation but show a heterogeneous expression profile of activation and effector molecules, isolation of tumour-reactive T cells based on single activation or effector molecules could result in the absence of tumour-reactive T cells; thus, combinations of multiple activation and effector molecules could improve the efficiency of isolating tumour-specific TCRs. We enrolled two patients with lung adenocarcinoma and obtained tCancer immunology, immunotherapy : CIISingle-cell sequencing of tumour infiltrating T cells efficiently identifies tumour-specific T cell receptors based on the T cell activation score.PMC1108738320230484366BMU2023YFJHPY011ZDYF2024SHF208782373248Shen LYang WLi SXiao YLu ZTeng XZhang CfalseSingle-cell sequencing of tumour infiltrating T cells efficiently identifies tumour-specific T cell receptors based on the T cell activation score.Adoptively transferred T cell receptor-engineered T cells are a promising cancer treatment strategy, and the identification of tumour-specific TCRs is essential. Previous studies reported that tumour-reactive T cells and TCRs could be isolated based on the expression of activation markers. However, since T cells with different cell states could not respond uniformly to activation but show a heterogeneous expression profile of activation and effector molecules, isolation of tumour-reactive T cells based on single activation or effector molecules could result in the absence of tumour-reactive T cells; thus, combinations of multiple activation and effector molecules could improve the efficiency of isolating tumour-specific TCRs. We enrolled two patients with lung adenocarcinoma and obtained t2024-01-01T00:00:00Z2024 May2026-04-16T03:19:33.669Z2026-04-16T03:11:03.658ZS-EPMC110873833872781210.1007/s00262-024-03710-9