{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kotov A"],"funding":["HHS | National Institutes of Health","EC | Horizon Europe | Excellent Science | HORIZON EUROPE Marie Sklodowska-Curie Actions","NICHD NIH HHS","Agence Nationale de la Recherche","HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","Institut Universitaire de France","NIGMS NIH HHS","NIH HHS"],"pagination":["e2311685121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11087755"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(19)"],"pubmed_abstract":["Neural crest cells exemplify cellular diversification from a multipotent progenitor population. However, the full sequence of early molecular choices orchestrating the emergence of neural crest heterogeneity from the embryonic ectoderm remains elusive. Gene-regulatory-networks (GRN) govern early development and cell specification toward definitive neural crest. Here, we combine ultradense single-cell transcriptomes with machine-learning and large-scale transcriptomic and epigenomic experimental validation of selected trajectories, to provide the general principles and highlight specific features of the GRN underlying neural crest fate diversification from induction to early migration stages using <i>Xenopus</i> frog embryos as a model. During gastrulation, a transient neural border zone st"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages."],"pmcid":["PMC11087755"],"funding_grant_id":["R24 OD031956","R01 GM042341","ANR-21-CE13-0028","860635 NEUcrest","NA","R01 GM42341","R01 HD073104","R01HD073104","R35 GM127069","R35GM127069","ANR-15-CE13-0012-01"],"pubmed_authors":["Peshkin L","Alkobtawi M","Ruiz SM","Monsoro-Burq AH","Arbes H","Kappes V","Harland RM","Kotov A","Seal S"],"additional_accession":[]},"is_claimable":false,"name":"A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages.","description":"Neural crest cells exemplify cellular diversification from a multipotent progenitor population. However, the full sequence of early molecular choices orchestrating the emergence of neural crest heterogeneity from the embryonic ectoderm remains elusive. Gene-regulatory-networks (GRN) govern early development and cell specification toward definitive neural crest. Here, we combine ultradense single-cell transcriptomes with machine-learning and large-scale transcriptomic and epigenomic experimental validation of selected trajectories, to provide the general principles and highlight specific features of the GRN underlying neural crest fate diversification from induction to early migration stages using <i>Xenopus</i> frog embryos as a model. During gastrulation, a transient neural border zone st","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-03T05:26:11.821Z","creation":"2025-04-04T02:59:07.059Z"},"accession":"S-EPMC11087755","cross_references":{"pubmed":["38683994"],"doi":["10.1073/pnas.2311685121"]}}