{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["33(8)"],"submitter":["Zhang Q"],"pubmed_abstract":["<i>Akkermansia muciniphila</i> (<i>Akk</i>) has recently become popular due to its therapeutic effect on various diseases. However, <i>Akk</i>'s high-density cultivation is difficult due to its anaerobic characteristics. Therefore, <i>Akk</i> was cultured with modified brain-heart infusion (M-BHI) to reach 10<sup>11</sup> CFU/mL. <sup>1</sup>H-NMR determined the metabolites of <i>Akk</i> and validated them by an amino acid analyzer. Compared to the BHI, <i>Akk</i> significantly up-regulated lactate, histidine, fumaric acid, cytidine, threonine, arginine, and hydroxyproline in the M-BHI and significantly down-regulated methionine, trimethylamine, and sarcosine. Regarding pathway enrichment analysis, histidine metabolism, arginine and proline metabolism, cysteine and methionine metabolism mainly regulate differential metabolites. In addition, M-BHI alters the metabolic profile by affecting <i>Akk</i>'s involvement in amino acid metabolism remodeling. Changed metabolites showed that <i>Akk</i> fermentation in M-BHI may play a physiological role in regulating immune homeostasis and reducing risk factors related to diseases. Therefore, M-BHI provides a promising reference for <i>Akk</i> cultivation in future industrial preparation.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s10068-023-01492-x."],"journal":["Food science and biotechnology"],"pagination":["1921-1930"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11091034"],"repository":["biostudies-literature"],"pubmed_title":["Effects of modified-BHI medium on the growth and metabolites of &lt;i&gt;Akkermansia muciniphila&lt;/i&gt;."],"pmcid":["PMC11091034"],"pubmed_authors":["Chi P","Li Q","Zhou F","Zhou Y","He Q","Zhang Q","Zhao H"],"additional_accession":[]},"is_claimable":false,"name":"Effects of modified-BHI medium on the growth and metabolites of &lt;i&gt;Akkermansia muciniphila&lt;/i&gt;.","description":"<i>Akkermansia muciniphila</i> (<i>Akk</i>) has recently become popular due to its therapeutic effect on various diseases. However, <i>Akk</i>'s high-density cultivation is difficult due to its anaerobic characteristics. Therefore, <i>Akk</i> was cultured with modified brain-heart infusion (M-BHI) to reach 10<sup>11</sup> CFU/mL. <sup>1</sup>H-NMR determined the metabolites of <i>Akk</i> and validated them by an amino acid analyzer. Compared to the BHI, <i>Akk</i> significantly up-regulated lactate, histidine, fumaric acid, cytidine, threonine, arginine, and hydroxyproline in the M-BHI and significantly down-regulated methionine, trimethylamine, and sarcosine. Regarding pathway enrichment analysis, histidine metabolism, arginine and proline metabolism, cysteine and methionine metabolism mainly regulate differential metabolites. In addition, M-BHI alters the metabolic profile by affecting <i>Akk</i>'s involvement in amino acid metabolism remodeling. Changed metabolites showed that <i>Akk</i> fermentation in M-BHI may play a physiological role in regulating immune homeostasis and reducing risk factors related to diseases. Therefore, M-BHI provides a promising reference for <i>Akk</i> cultivation in future industrial preparation.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s10068-023-01492-x.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-04T01:24:32.661Z","creation":"2025-04-04T01:24:32.661Z"},"accession":"S-EPMC11091034","cross_references":{"pubmed":["38752110"],"doi":["10.1007/s10068-023-01492-x"]}}