biostudies-literatureMarbun KTInstitute of Research and Community Service of Universitas Pelita Harapan871-879https://www.ebi.ac.uk/biostudies/studies/S-EPMC11091697biostudies-literatureUnknown34(4)Our group had isolated <i>Bifidobacterium breve</i> strain BS2-PB3 from human breast milk. In this study, we sequenced the whole genome of <i>B. breve</i> BS2-PB3, and with a focus on its safety profile, various probiotic characteristics (presence of antibiotic resistance genes, virulence factors, and mobile elements) were then determined through bioinformatic analyses. The antibiotic resistance profile of <i>B. breve</i> BS2-PB3 was also evaluated. The whole genome of <i>B. breve</i> BS2-PB3 consisted of 2,268,931 base pairs with a G-C content of 58.89% and 2,108 coding regions. The average nucleotide identity and whole-genome phylogenetic analyses supported the classification of <i>B. breve</i> BS2-PB3. According to our in silico assessment, <i>B. breve</i> BS2-PB3 possesses antioxidant Journal of microbiology and biotechnologyGenomic Characterization and Safety Assessment of <i>Bifidobacterium breve</i> BS2-PB3 as Functional Food.PMC11091697P-01-FaST/I/2023P-14-FaST/VIII/2022Purnomo JSMarbun KTJan TTMudana SOSugata MJo JDiksonfalseGenomic Characterization and Safety Assessment of <i>Bifidobacterium breve</i> BS2-PB3 as Functional Food.Our group had isolated <i>Bifidobacterium breve</i> strain BS2-PB3 from human breast milk. In this study, we sequenced the whole genome of <i>B. breve</i> BS2-PB3, and with a focus on its safety profile, various probiotic characteristics (presence of antibiotic resistance genes, virulence factors, and mobile elements) were then determined through bioinformatic analyses. The antibiotic resistance profile of <i>B. breve</i> BS2-PB3 was also evaluated. The whole genome of <i>B. breve</i> BS2-PB3 consisted of 2,268,931 base pairs with a G-C content of 58.89% and 2,108 coding regions. The average nucleotide identity and whole-genome phylogenetic analyses supported the classification of <i>B. breve</i> BS2-PB3. According to our in silico assessment, <i>B. breve</i> BS2-PB3 possesses antioxidant 2024-01-01T00:00:00Z2024 Apr2026-06-02T06:09:16.835Z2026-04-15T03:10:07.154ZS-EPMC110916973849488410.4014/jmb.2311.11031