biostudies-literatureVaknin AUnited States-Israel Binational Science FoundationEuropean Research CouncilNIAID NIH HHSIsrael Science FoundationNIGMS NIH HHSNational Science Foundation1590-1601https://www.ebi.ac.uk/biostudies/studies/S-EPMC11091876biostudies-literatureUnknown10(5)Ebola virus (EBOV) is an enveloped virus that must fuse with the host cell membrane in order to release its genome and initiate infection. This process requires the action of the EBOV envelope glycoprotein (GP), encoded by the virus, which resides in the viral envelope and consists of a receptor binding subunit, GP1, and a membrane fusion subunit, GP2. Despite extensive research, a mechanistic understanding of the viral fusion process is incomplete. To investigate GP-membrane association, a key step in the fusion process, we used two approaches: high-throughput measurements of single-particle diffusion and single-molecule measurements with optical tweezers. Using these methods, we show that the presence of the endosomal Niemann-Pick C1 (NPC1) receptor is not required for primed GP-membraneACS infectious diseasesEbola Virus Glycoprotein Strongly Binds to Membranes in the Absence of Receptor Engagement.PMC1109187620217931010775021289/20R01 AI174645R01 GM143773R01 AI148784Vaknin AGolani GGrossman AMunro JBGoren SDurham NDRoichman YSorkin RLupovitz IfalseEbola Virus Glycoprotein Strongly Binds to Membranes in the Absence of Receptor Engagement.Ebola virus (EBOV) is an enveloped virus that must fuse with the host cell membrane in order to release its genome and initiate infection. This process requires the action of the EBOV envelope glycoprotein (GP), encoded by the virus, which resides in the viral envelope and consists of a receptor binding subunit, GP1, and a membrane fusion subunit, GP2. Despite extensive research, a mechanistic understanding of the viral fusion process is incomplete. To investigate GP-membrane association, a key step in the fusion process, we used two approaches: high-throughput measurements of single-particle diffusion and single-molecule measurements with optical tweezers. Using these methods, we show that the presence of the endosomal Niemann-Pick C1 (NPC1) receptor is not required for primed GP-membrane2024-01-01T00:00:00Z2024 May2026-06-02T06:06:12.129Z2026-04-15T03:09:48.778ZS-EPMC110918763868407310.1021/acsinfecdis.3c00622