{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["74(4)"],"submitter":["Sawada H"],"funding":["Environmental Restoration and Conservation Agency"],"pubmed_abstract":["Phytopathogenic bacteria (MAFF 302110<sup>T</sup> and MAFF 302107) were isolated from lesions on Japanese angelica trees affected by bacterial soft rot in Yamanashi Prefecture, Japan. The strains were Gram-reaction-negative, facultatively anaerobic, motile with peritrichous flagella, rod-shaped, and non-spore-forming. The genomic DNA G+C content was 51.1 mol % and the predominant cellular fatty acids included summed feature 3 (C<sub>16 : 1</sub> ω7<i>c</i> and/or C<sub>16 : 1</sub> ω6<i>c</i>), C<sub>16 : 0</sub>, summed feature 8 (C<sub>18 : 1</sub> ω7<i>c</i> and/or C<sub>18 : 1</sub> ω6<i>c</i>), summed feature 2 (comprising any combination of C<sub>12 : 0</sub> aldehyde, an unknown fatty acid with an equivalent chain length of 10.928, C<sub>16 : 1</sub> iso I, and C<sub>14 : 0</sub> 3OH), and C<sub>12 : 0</sub>. Phylogenetic analyses based on 16S rRNA and <i>gyrB</i> gene sequences, along with phylogenomic analysis utilizing whole-genome sequences, consistently placed these strains within the genus <i>Pectobacterium</i>. However, their phylogenetic positions did not align with any known species within the genus. Comparative studies involving average nucleotide identity and digital DNA-DNA hybridization with the closely related species indicated values below the thresholds employed for the prokaryotic species delineation (95-96 % and 70 %, respectively), with the highest values observed for <i>Pectobacterium polonicum</i> DPMP315<sup>T</sup> (92.10 and 47.1 %, respectively). Phenotypic characteristics, cellular fatty acid composition, and a repertoire of secretion systems could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic, and genotypic data obtained in this study show that MAFF 302110<sup>T</sup>/MAFF 302107 represent a novel species of the genus <i>Pectobacterium</i>, for which we propose the name <i>Pectobacterium araliae</i> sp. nov., designating MAFF 302110<sup>T</sup> (=ICMP 25161<sup>T</sup>) as the type strain."],"journal":["International journal of systematic and evolutionary microbiology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11092084"],"repository":["biostudies-literature"],"pubmed_title":["<i>Pectobacterium araliae</i> sp. nov., a pathogen causing bacterial soft rot of Japanese angelica tree in Japan."],"pmcid":["PMC11092084"],"funding_grant_id":["S18-2"],"pubmed_authors":["Ono M","Someya N","Morohoshi T","Sawada H","Satou M"],"additional_accession":[]},"is_claimable":false,"name":"<i>Pectobacterium araliae</i> sp. nov., a pathogen causing bacterial soft rot of Japanese angelica tree in Japan.","description":"Phytopathogenic bacteria (MAFF 302110<sup>T</sup> and MAFF 302107) were isolated from lesions on Japanese angelica trees affected by bacterial soft rot in Yamanashi Prefecture, Japan. The strains were Gram-reaction-negative, facultatively anaerobic, motile with peritrichous flagella, rod-shaped, and non-spore-forming. The genomic DNA G+C content was 51.1 mol % and the predominant cellular fatty acids included summed feature 3 (C<sub>16 : 1</sub> ω7<i>c</i> and/or C<sub>16 : 1</sub> ω6<i>c</i>), C<sub>16 : 0</sub>, summed feature 8 (C<sub>18 : 1</sub> ω7<i>c</i> and/or C<sub>18 : 1</sub> ω6<i>c</i>), summed feature 2 (comprising any combination of C<sub>12 : 0</sub> aldehyde, an unknown fatty acid with an equivalent chain length of 10.928, C<sub>16 : 1</sub> iso I, and C<sub>14 : 0</sub> 3OH), and C<sub>12 : 0</sub>. Phylogenetic analyses based on 16S rRNA and <i>gyrB</i> gene sequences, along with phylogenomic analysis utilizing whole-genome sequences, consistently placed these strains within the genus <i>Pectobacterium</i>. However, their phylogenetic positions did not align with any known species within the genus. Comparative studies involving average nucleotide identity and digital DNA-DNA hybridization with the closely related species indicated values below the thresholds employed for the prokaryotic species delineation (95-96 % and 70 %, respectively), with the highest values observed for <i>Pectobacterium polonicum</i> DPMP315<sup>T</sup> (92.10 and 47.1 %, respectively). Phenotypic characteristics, cellular fatty acid composition, and a repertoire of secretion systems could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic, and genotypic data obtained in this study show that MAFF 302110<sup>T</sup>/MAFF 302107 represent a novel species of the genus <i>Pectobacterium</i>, for which we propose the name <i>Pectobacterium araliae</i> sp. nov., designating MAFF 302110<sup>T</sup> (=ICMP 25161<sup>T</sup>) as the type strain.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-01T20:15:48.182Z","creation":"2026-05-20T03:08:38.495Z"},"accession":"S-EPMC11092084","cross_references":{"pubmed":["38625720"],"doi":["10.1099/ijsem.0.006326"]}}