biostudies-literatureUnknown74(4)Sawada HEnvironmental Restoration and Conservation AgencyPhytopathogenic bacteria (MAFF 302110^T and MAFF 302107) were isolated from lesions on Japanese angelica trees affected by bacterial soft rot in Yamanashi Prefecture, Japan. The strains were Gram-reaction-negative, facultatively anaerobic, motile with peritrichous flagella, rod-shaped, and non-spore-forming. The genomic DNA G+C content was 51.1 mol % and the predominant cellular fatty acids included summed feature 3 (C_16 : 1 ω7<i>c</i> and/or C_16 : 1 ω6<i>c</i>), C_16 : 0, summed feature 8 (C_18 : 1 ω7<i>c</i> and/or C_18 : 1 ω6<i>c</i>), summed feature 2 (comprising any combination of C_12 : 0 aldehyde, an unknown fatty acid with an equivalent chain length of 10.928, C_16 : 1 iso I, and C_14 : 0 3OH), and C_12 : 0. Phylogenetic analyses based on 16S rRNA and <i>gyrB</i> gene sequences, along with phylogenomic analysis utilizing whole-genome sequences, consistently placed these strains within the genus <i>Pectobacterium</i>. However, their phylogenetic positions did not align with any known species within the genus. Comparative studies involving average nucleotide identity and digital DNA-DNA hybridization with the closely related species indicated values below the thresholds employed for the prokaryotic species delineation (95-96 % and 70 %, respectively), with the highest values observed for <i>Pectobacterium polonicum</i> DPMP315^T (92.10 and 47.1 %, respectively). Phenotypic characteristics, cellular fatty acid composition, and a repertoire of secretion systems could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic, and genotypic data obtained in this study show that MAFF 302110^T/MAFF 302107 represent a novel species of the genus <i>Pectobacterium</i>, for which we propose the name <i>Pectobacterium araliae</i> sp. nov., designating MAFF 302110^T (=ICMP 25161^T) as the type strain.International journal of systematic and evolutionary microbiologyhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC11092084biostudies-literature<i>Pectobacterium araliae</i> sp. nov., a pathogen causing bacterial soft rot of Japanese angelica tree in Japan.PMC11092084S18-2Ono MSomeya NMorohoshi TSawada HSatou Mfalse<i>Pectobacterium araliae</i> sp. nov., a pathogen causing bacterial soft rot of Japanese angelica tree in Japan.Phytopathogenic bacteria (MAFF 302110^T and MAFF 302107) were isolated from lesions on Japanese angelica trees affected by bacterial soft rot in Yamanashi Prefecture, Japan. The strains were Gram-reaction-negative, facultatively anaerobic, motile with peritrichous flagella, rod-shaped, and non-spore-forming. The genomic DNA G+C content was 51.1 mol % and the predominant cellular fatty acids included summed feature 3 (C_16 : 1 ω7<i>c</i> and/or C_16 : 1 ω6<i>c</i>), C_16 : 0, summed feature 8 (C_18 : 1 ω7<i>c</i> and/or C_18 : 1 ω6<i>c</i>), summed feature 2 (comprising any combination of C_12 : 0 aldehyde, an unknown fatty acid with an equivalent chain length of 10.928, C_16 : 1 iso I, and C_14 : 0 3OH), and C_12 : 0. Phylogenetic analyses based on 16S rRNA and <i>gyrB</i> gene sequences, along with phylogenomic analysis utilizing whole-genome sequences, consistently placed these strains within the genus <i>Pectobacterium</i>. However, their phylogenetic positions did not align with any known species within the genus. Comparative studies involving average nucleotide identity and digital DNA-DNA hybridization with the closely related species indicated values below the thresholds employed for the prokaryotic species delineation (95-96 % and 70 %, respectively), with the highest values observed for <i>Pectobacterium polonicum</i> DPMP315^T (92.10 and 47.1 %, respectively). Phenotypic characteristics, cellular fatty acid composition, and a repertoire of secretion systems could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic, and genotypic data obtained in this study show that MAFF 302110^T/MAFF 302107 represent a novel species of the genus <i>Pectobacterium</i>, for which we propose the name <i>Pectobacterium araliae</i> sp. nov., designating MAFF 302110^T (=ICMP 25161^T) as the type strain.2024-01-01T00:00:00Z2024 Apr2026-06-01T20:15:48.182Z2026-05-20T03:08:38.495ZS-EPMC110920843862572010.1099/ijsem.0.006326