{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Benning FMC"],"funding":["Natural Sciences and Engineering Research Council of Canada","Swiss National Science Foundation","Moore–Simons Foundations","Canada Foundation for Innovation","NIGMS NIH HHS","Canadian Institutes of Health Research","HHWF","NIGMS","New Frontiers in Research Fund-Exploration"],"pubmed_abstract":["In eukaryotes, cellular respiration takes place in the cristae of mitochondria. The mitochondrial inner membrane protein Mic60, a core component of the mitochondrial contact site and cristae organizing system (MICOS), is crucial for the organization and stabilization of crista junctions and its associated functions. While the C-terminal Mitofilin domain of Mic60 is necessary for cellular respiration, the sequence determinants for this function have remained unclear. Here, we used ancestral sequence reconstruction to generate Mitofilin ancestors up to and including the last opisthokont common ancestor (LOCA). We found that yeast-lineage derived Mitofilin ancestors as far back as the LOCA rescue respiration. By comparing Mitofilin ancestors, we identified four residues sufficient to explain "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.04.26.591372"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11092495"],"repository":["biostudies-literature"],"pubmed_title":["Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast."],"pmcid":["PMC11092495"],"funding_grant_id":["P180777","34475","377068","NFRFE- 2018-00064","R35 GM142553","9736","R35GM142553","RGPIN-2016- 04801"],"pubmed_authors":["Liao YT","Nordstrom AEH","Bell TA","Nguyen TH","Syau D","daCosta CJB","Chao LH","Benning FMC","Ericsson M","Connell LB","Keating MP","Coughlin M"],"additional_accession":[]},"is_claimable":false,"name":"Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast.","description":"In eukaryotes, cellular respiration takes place in the cristae of mitochondria. The mitochondrial inner membrane protein Mic60, a core component of the mitochondrial contact site and cristae organizing system (MICOS), is crucial for the organization and stabilization of crista junctions and its associated functions. While the C-terminal Mitofilin domain of Mic60 is necessary for cellular respiration, the sequence determinants for this function have remained unclear. Here, we used ancestral sequence reconstruction to generate Mitofilin ancestors up to and including the last opisthokont common ancestor (LOCA). We found that yeast-lineage derived Mitofilin ancestors as far back as the LOCA rescue respiration. By comparing Mitofilin ancestors, we identified four residues sufficient to explain ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-05-23T03:19:27.365Z","creation":"2025-04-04T01:40:17.736Z"},"accession":"S-EPMC11092495","cross_references":{"pubmed":["38746426"],"doi":["10.1101/2024.04.26.591372"]}}