biostudies-literatureUnknownKwon SNIGMS NIH HHSEnzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and co-localization of enzymes can increase catalytic activity, stability, and longevity, highly desirable features for many biotechnological and biomedical applications of enzyme catalysts. One promising strategy to construct enzyme nanoreactors is to repurpose protein nanocages found in nature. However, protein-based enzyme nanoreactors often exhibit decreased catalytic activity, partially caused by a mismatch of protein shell selectivity and the substrate requirements of encapsulated enzymes. No broadly applicable and modular protein-based nanoreactor platform is currently available. Here, we introduce a pore-engineered universal enzyme nanoreactor platforbioRxiv : the preprint server for biology2024.05.02.592161https://www.ebi.ac.uk/biostudies/studies/S-EPMC11092584biostudies-literaturePore engineering as a general strategy to improve protein-based enzyme nanoreactor performance.PMC11092584R01 GM129325R35 GM133325Kwon SGiessen TWAndreas MPfalsePore engineering as a general strategy to improve protein-based enzyme nanoreactor performance.Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and co-localization of enzymes can increase catalytic activity, stability, and longevity, highly desirable features for many biotechnological and biomedical applications of enzyme catalysts. One promising strategy to construct enzyme nanoreactors is to repurpose protein nanocages found in nature. However, protein-based enzyme nanoreactors often exhibit decreased catalytic activity, partially caused by a mismatch of protein shell selectivity and the substrate requirements of encapsulated enzymes. No broadly applicable and modular protein-based nanoreactor platform is currently available. Here, we introduce a pore-engineered universal enzyme nanoreactor platfor2024-01-01T00:00:00Z2024 May2026-05-26T20:12:33.551Z2026-05-26T03:07:28.948ZS-EPMC110925843874612710.1101/2024.05.02.592161