{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ootsuki S"],"funding":["Japan Agency for Medical Research and Development","Japan Society for the Promotion of Science"],"pagination":["11055"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11094154"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Identifying pathogenic microorganisms as early as possible is critical for selecting the appropriate antimicrobial therapy in infected patients. We previously reported the development of the Tm mapping method for identifying a broad range of pathogenic bacteria within 3 h of blood collection. However, the Tm mapping identification requires an analytical instrument with a tube-to-tube variation of no more than 0.1 °C, so we can only use a few instruments that have such high thermal accuracy. To address the problem, we developed the improved Tm mapping method using imperfect-match linear long quenching probes (IMLL Q-probes). Using IMLL Q-probes, almost all commercially available analytical instruments can be used for the Tm mapping method. Some bacterial species cannot be narrowed down to o"],"journal":["Scientific reports"],"pubmed_title":["Melting temperature mapping method using imperfect-match linear long probes."],"pmcid":["PMC11094154"],"funding_grant_id":["KAKENHI 21H02825","KAKENHI 18H02728","JP19im0210217"],"pubmed_authors":["Mori M","Kitajima I","Ootsuki S","Minami H","Tabata H","Ueno T","Niimi H"],"additional_accession":[]},"is_claimable":false,"name":"Melting temperature mapping method using imperfect-match linear long probes.","description":"Identifying pathogenic microorganisms as early as possible is critical for selecting the appropriate antimicrobial therapy in infected patients. We previously reported the development of the Tm mapping method for identifying a broad range of pathogenic bacteria within 3 h of blood collection. However, the Tm mapping identification requires an analytical instrument with a tube-to-tube variation of no more than 0.1 °C, so we can only use a few instruments that have such high thermal accuracy. To address the problem, we developed the improved Tm mapping method using imperfect-match linear long quenching probes (IMLL Q-probes). Using IMLL Q-probes, almost all commercially available analytical instruments can be used for the Tm mapping method. Some bacterial species cannot be narrowed down to o","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-01T10:59:46.581Z","creation":"2026-04-08T11:31:49.11Z"},"accession":"S-EPMC11094154","cross_references":{"pubmed":["38745038"],"doi":["10.1038/s41598-024-60987-7"]}}