biostudies-literatureOotsuki SJapan Agency for Medical Research and DevelopmentJapan Society for the Promotion of Science11055https://www.ebi.ac.uk/biostudies/studies/S-EPMC11094154biostudies-literatureUnknown14(1)Identifying pathogenic microorganisms as early as possible is critical for selecting the appropriate antimicrobial therapy in infected patients. We previously reported the development of the Tm mapping method for identifying a broad range of pathogenic bacteria within 3 h of blood collection. However, the Tm mapping identification requires an analytical instrument with a tube-to-tube variation of no more than 0.1 °C, so we can only use a few instruments that have such high thermal accuracy. To address the problem, we developed the improved Tm mapping method using imperfect-match linear long quenching probes (IMLL Q-probes). Using IMLL Q-probes, almost all commercially available analytical instruments can be used for the Tm mapping method. Some bacterial species cannot be narrowed down to oScientific reportsMelting temperature mapping method using imperfect-match linear long probes.PMC11094154KAKENHI 21H02825KAKENHI 18H02728JP19im0210217Mori MKitajima IOotsuki SMinami HTabata HUeno TNiimi HfalseMelting temperature mapping method using imperfect-match linear long probes.Identifying pathogenic microorganisms as early as possible is critical for selecting the appropriate antimicrobial therapy in infected patients. We previously reported the development of the Tm mapping method for identifying a broad range of pathogenic bacteria within 3 h of blood collection. However, the Tm mapping identification requires an analytical instrument with a tube-to-tube variation of no more than 0.1 °C, so we can only use a few instruments that have such high thermal accuracy. To address the problem, we developed the improved Tm mapping method using imperfect-match linear long quenching probes (IMLL Q-probes). Using IMLL Q-probes, almost all commercially available analytical instruments can be used for the Tm mapping method. Some bacterial species cannot be narrowed down to o2024-01-01T00:00:00Z2024 May2026-06-01T10:59:46.581Z2026-04-08T11:31:49.11ZS-EPMC110941543874503810.1038/s41598-024-60987-7