biostudies-literatureKawaai KAcademic development project Grant-in-aid from JSRJapan Society for the Promotion of Science2983-2994https://www.ebi.ac.uk/biostudies/studies/S-EPMC11094677biostudies-literatureUnknown10(5)Calcified cartilage digested by chondroclasts provides an excellent scaffold to initiate bone formation. We analyzed bioactive proteins and microarchitecture of calcified cartilage either separately or in combination and evaluated biomimetic osteogenic culture conditions of surface-coated micropatterning. To do so, we prepared a crude extract from porcine femoral growth plates, which enhanced <i>in vitro</i> mineralization when coated on flat-bottom culture dishes, and identified four candidate proteins by fractionation and mass spectrometry. Murine homologues of two candidates, desmoglein 4 (DSG4) and peroxiredoxin 6 (PRDX6), significantly promoted osteogenic activity based on <i>in vitro</i> mineralization and osteoblast differentiation. Moreover, we observed DSG4 and PRDX6 protein expreACS biomaterials science & engineeringCalcified Cartilage-Guided Identification of Osteogenic Molecules and Geometries.PMC1109467719K12807Kuroda YKawaai KMatsuo KfalseCalcified Cartilage-Guided Identification of Osteogenic Molecules and Geometries.Calcified cartilage digested by chondroclasts provides an excellent scaffold to initiate bone formation. We analyzed bioactive proteins and microarchitecture of calcified cartilage either separately or in combination and evaluated biomimetic osteogenic culture conditions of surface-coated micropatterning. To do so, we prepared a crude extract from porcine femoral growth plates, which enhanced <i>in vitro</i> mineralization when coated on flat-bottom culture dishes, and identified four candidate proteins by fractionation and mass spectrometry. Murine homologues of two candidates, desmoglein 4 (DSG4) and peroxiredoxin 6 (PRDX6), significantly promoted osteogenic activity based on <i>in vitro</i> mineralization and osteoblast differentiation. Moreover, we observed DSG4 and PRDX6 protein expre2024-01-01T00:00:00Z2024 May2026-06-01T11:18:41.541Z2026-04-08T11:46:15.273ZS-EPMC110946773863461510.1021/acsbiomaterials.3c01799