biostudies-literatureLao ZNational Medical Research Council (NMRC)NCI NIH HHSNational Medical Research Council2170-2180https://www.ebi.ac.uk/biostudies/studies/S-EPMC11096012biostudies-literatureUnknown30(10)<h4>Purpose</h4>DNA methylation alterations are widespread in acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS), some of which appear to have evolved independently of somatic mutations in epigenetic regulators. Although the presence of somatic mutations in peripheral blood can predict the risk of development of AML and MDS, its accuracy remains unsatisfactory.<h4>Experimental design</h4>We performed global DNA methylation profiling in a case control study nested within the Singapore Chinese Health Study to evaluate whether DNA methylation alterations were associated with AML/MDS development. Targeted deep sequencing and methylated DNA immunoprecipitation sequencing (MeDIP-seq) were performed on peripheral blood collected a median of 9.9 years before diagnosis of AML or MDClinical cancer research : an official journal of the American Association for Cancer ResearchA Pre-Leukemic DNA Methylation Signature in Healthy Individuals at Higher Risk for Developing Myeloid Malignancy.PMC11096012R01 CA144034UM1 CA182876MOH-OFIRG21nov-0007NMRC/Fellowship/0036/2016NMRC/TA/0051/2016Yang HYuan JMChng WJNg AYLao ZGrigoropoulos NKoeffler HPJia LYing LGoh YTKoh WPWang RSun QYYan BCapinpin SMDing LWfalseA Pre-Leukemic DNA Methylation Signature in Healthy Individuals at Higher Risk for Developing Myeloid Malignancy.<h4>Purpose</h4>DNA methylation alterations are widespread in acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS), some of which appear to have evolved independently of somatic mutations in epigenetic regulators. Although the presence of somatic mutations in peripheral blood can predict the risk of development of AML and MDS, its accuracy remains unsatisfactory.<h4>Experimental design</h4>We performed global DNA methylation profiling in a case control study nested within the Singapore Chinese Health Study to evaluate whether DNA methylation alterations were associated with AML/MDS development. Targeted deep sequencing and methylated DNA immunoprecipitation sequencing (MeDIP-seq) were performed on peripheral blood collected a median of 9.9 years before diagnosis of AML or MD2024-01-01T00:00:00Z2024 May2026-06-03T05:17:11.64Z2025-04-04T14:50:57.483ZS-EPMC110960123843767910.1158/1078-0432.CCR-22-380410.1158/1078-0432.ccr-22-3804