biostudies-literaturePandey RAdvancing a Healthier Wisconsin EndowmentNHLBI NIH HHSNational Institutes of Health102036https://www.ebi.ac.uk/biostudies/studies/S-EPMC11098693biostudies-literatureUnknown104(5)Arterioles are key determinants of the total peripheral vascular resistance, which, in turn, is a key determinant of arterial blood pressure. However, the amount of protein available from one isolated human arteriole may be less than 5 μg, making proteomic analysis challenging. In addition, obtaining human arterioles requires manual dissection of unfrozen clinical specimens. This limits its feasibility, especially for powerful multicenter clinical studies in which clinical specimens need to be shipped overnight to a research laboratory for arteriole isolation. We performed a study to address low-input, test overnight tissue storage and develop a reference human arteriolar proteomic profile. In tandem mass tag proteomics, use of a booster channel consisting of human induced pluripotent stemLaboratory investigation; a journal of technical methods and pathologyProteomic Profiles of Human Arterioles Isolated From Fresh Adipose Tissue or Following Overnight Storage.PMC11098693HL149620R38 HL167238HL144098K24 HL152143K24152143P01 HL149620HL121233R38 HL143561R01 HL144098R01 HL121233Pereckas MRoberts MLJensen DLiang MGreene ASPandey RWang JWidlansky MEfalseProteomic Profiles of Human Arterioles Isolated From Fresh Adipose Tissue or Following Overnight Storage.Arterioles are key determinants of the total peripheral vascular resistance, which, in turn, is a key determinant of arterial blood pressure. However, the amount of protein available from one isolated human arteriole may be less than 5 μg, making proteomic analysis challenging. In addition, obtaining human arterioles requires manual dissection of unfrozen clinical specimens. This limits its feasibility, especially for powerful multicenter clinical studies in which clinical specimens need to be shipped overnight to a research laboratory for arteriole isolation. We performed a study to address low-input, test overnight tissue storage and develop a reference human arteriolar proteomic profile. In tandem mass tag proteomics, use of a booster channel consisting of human induced pluripotent stem2024-01-01T00:00:00Z2024 May2025-07-03T03:04:55.369Z2025-07-03T03:04:55.369ZS-EPMC110986933840870410.1016/j.labinv.2024.102036