{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Scarfo R"],"funding":["European Research Council","Telethon","NHLBI NIH HHS","Agence Nationale de la Recherche (French National Research Agency)","Associazione Italiana per la Ricerca sul Cancro (Italian Association for Cancer Research)","U.S. Department of Health &amp; Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)"],"pagination":["719-730"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11098737"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(5)"],"pubmed_abstract":["During embryonic development, blood cells emerge from specialized endothelial cells, named haemogenic endothelial cells (HECs). As HECs are rare and only transiently found in early developing embryos, it remains difficult to distinguish them from endothelial cells. Here we performed transcriptomic analysis of 28- to 32-day human embryos and observed that the expression of Fc receptor CD32 (FCGR2B) is highly enriched in the endothelial cell population that contains HECs. Functional analyses using human embryonic and human pluripotent stem cell-derived endothelial cells revealed that robust multilineage haematopoietic potential is harboured within CD32<sup>+</sup> endothelial cells and showed that 90% of CD32<sup>+</sup> endothelial cells are bona fide HECs. Remarkably, these analyses indica"],"journal":["Nature cell biology"],"pubmed_title":["CD32 captures committed haemogenic endothelial cells during human embryonic development."],"pmcid":["PMC11098737"],"funding_grant_id":["101044032","TGT16C04","26779-2021","TGT16G03B","TTADC0422TT","R01 HL151777","ANR-14-CE11-0008","R01HL151777","R01 HL145290"],"pubmed_authors":["Randolph LN","Villa A","Gregori S","Freund JN","Ferrari Ramondo G","Luff SA","Tavian M","Sturgeon CM","Cascione S","El Khoury M","Ditadi A","Gersch A","Valsoni S","Brandas C","Merelli I","Abou Alezz M","Scarfo R","Tavosanis A","Passerini L","Li ZY","Didelon E","Amodio G"],"additional_accession":[]},"is_claimable":false,"name":"CD32 captures committed haemogenic endothelial cells during human embryonic development.","description":"During embryonic development, blood cells emerge from specialized endothelial cells, named haemogenic endothelial cells (HECs). As HECs are rare and only transiently found in early developing embryos, it remains difficult to distinguish them from endothelial cells. Here we performed transcriptomic analysis of 28- to 32-day human embryos and observed that the expression of Fc receptor CD32 (FCGR2B) is highly enriched in the endothelial cell population that contains HECs. Functional analyses using human embryonic and human pluripotent stem cell-derived endothelial cells revealed that robust multilineage haematopoietic potential is harboured within CD32<sup>+</sup> endothelial cells and showed that 90% of CD32<sup>+</sup> endothelial cells are bona fide HECs. Remarkably, these analyses indica","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-01T20:16:58.725Z","creation":"2026-05-20T03:08:50.485Z"},"accession":"S-EPMC11098737","cross_references":{"pubmed":["38594587"],"doi":["10.1038/s41556-024-01403-0"]}}