{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gilchrist JJ"],"funding":["Wellcome Trust"],"pagination":["100541"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11099345"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(5)"],"pubmed_abstract":["To better understand inter-individual variation in sensitivity of DNA methylation (DNAm) to immune activity, we characterized effects of inflammatory stimuli on primary monocyte DNAm (n = 190). We find that monocyte DNAm is site-dependently sensitive to lipopolysaccharide (LPS), with LPS-induced demethylation occurring following hydroxymethylation. We identify 7,359 high-confidence immune-modulated CpGs (imCpGs) that differ in genomic localization and transcription factor usage according to whether they represent a gain or loss in DNAm. Demethylated imCpGs are profoundly enriched for enhancers and colocalize to genes enriched for disease associations, especially cancer. DNAm is age associated, and we find that 24-h LPS exposure triggers approximately 6 months of gain in epigenetic age, dir"],"journal":["Cell genomics"],"pubmed_title":["Characterization of the genetic determinants of context-specific DNA methylation in primary monocytes."],"pmcid":["PMC11099345"],"funding_grant_id":["203141/Z/16/Z","204969/Z/16/Z","201488/Z/16/Z","090532/Z/09/Z"],"pubmed_authors":["Fang H","Knight JC","Nassiri I","Gilchrist JJ","Tong O","Schuster-Boeckler B","Muldoon D","Tomkova M","Neville M","Danielli S","Cohen LRZ","Fairfax BP","Al-Mossawi H","Taylor C","Lau E","Ng E"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of the genetic determinants of context-specific DNA methylation in primary monocytes.","description":"To better understand inter-individual variation in sensitivity of DNA methylation (DNAm) to immune activity, we characterized effects of inflammatory stimuli on primary monocyte DNAm (n = 190). We find that monocyte DNAm is site-dependently sensitive to lipopolysaccharide (LPS), with LPS-induced demethylation occurring following hydroxymethylation. We identify 7,359 high-confidence immune-modulated CpGs (imCpGs) that differ in genomic localization and transcription factor usage according to whether they represent a gain or loss in DNAm. Demethylated imCpGs are profoundly enriched for enhancers and colocalize to genes enriched for disease associations, especially cancer. DNAm is age associated, and we find that 24-h LPS exposure triggers approximately 6 months of gain in epigenetic age, dir","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-02T02:15:14.633Z","creation":"2026-04-13T03:12:59.419Z"},"accession":"S-EPMC11099345","cross_references":{"pubmed":["38663408"],"doi":["10.1016/j.xgen.2024.100541"]}}