{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lepore T"],"funding":["biotechnology and biological sciences research council"],"pagination":["418-427"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11110786"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["36(3)"],"pubmed_abstract":["Neosporosis and toxoplasmosis are major causes of abortion in livestock worldwide, leading to substantial economic losses. Detection tools are fundamental to the diagnosis and management of those diseases. Current immunohistochemistry (IHC) tests, using sera raised against whole parasite lysates, have not been able to distinguish between <i>Toxoplasma gondii</i> and <i>Neospora caninum.</i> We used <i>T. gondii</i> and <i>N. caninum</i> recombinant proteins, expressed in <i>Escherichia coli</i> and purified using insoluble conditions, to produce specific polyclonal rabbit antisera. We aimed to develop species-specific sera that could be used in IHC on formalin-fixed, paraffin-embedded (FFPE) tissue sections to improve the diagnosis of ruminant abortions caused by protozoa. Two polyclonal r"],"journal":["Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc"],"pubmed_title":["Evaluation of species-specific polyclonal antibodies to detect and differentiate between <i>Neospora caninum</i> and <i>Toxoplasma gondii</i>."],"pmcid":["PMC11110786"],"funding_grant_id":["1533233"],"pubmed_authors":["Palarea-Albaladejo J","Lepore T","Katzer F","Chianini F","Cantile C","Canton GJ","Martineau HM","Cahalan S","Macrae AI","Underwood C"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of species-specific polyclonal antibodies to detect and differentiate between <i>Neospora caninum</i> and <i>Toxoplasma gondii</i>.","description":"Neosporosis and toxoplasmosis are major causes of abortion in livestock worldwide, leading to substantial economic losses. Detection tools are fundamental to the diagnosis and management of those diseases. Current immunohistochemistry (IHC) tests, using sera raised against whole parasite lysates, have not been able to distinguish between <i>Toxoplasma gondii</i> and <i>Neospora caninum.</i> We used <i>T. gondii</i> and <i>N. caninum</i> recombinant proteins, expressed in <i>Escherichia coli</i> and purified using insoluble conditions, to produce specific polyclonal rabbit antisera. We aimed to develop species-specific sera that could be used in IHC on formalin-fixed, paraffin-embedded (FFPE) tissue sections to improve the diagnosis of ruminant abortions caused by protozoa. Two polyclonal r","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-02T19:16:20.07Z","creation":"2025-04-04T02:48:01.007Z"},"accession":"S-EPMC11110786","cross_references":{"pubmed":["38420701"],"doi":["10.1177/10406387241234322"]}}