biostudies-literatureUnknown11(1)Huang WFicus species (Moraceae) play pivotal roles in tropical and subtropical ecosystems. Thriving across diverse habitats, from rainforests to deserts, they harbor a multitude of mutualistic and antagonistic interactions with insects, nematodes, and pathogens. Despite their ecological significance, knowledge about the genomic background of Ficus remains limited. In this study, we report a chromosome-level reference genome of F. hirta, with a total size of 297.27 Mb, containing 28,625 protein-coding genes and 44.67% repeat sequences. These findings illuminate the genetic basis of Ficus responses to environmental challenges, offering valuable genomic resources for understanding genome size, adaptive evolution, and co-evolution with natural enemies and mutualists within the genus.Scientific data526https://www.ebi.ac.uk/biostudies/studies/S-EPMC11111794biostudies-literatureA high-quality chromosome-level genome assembly of Ficus hirta.PMC11111794Ding YHuang WChen HFan SSegar SYu HLiu WCompton SGfalseA high-quality chromosome-level genome assembly of Ficus hirta.Ficus species (Moraceae) play pivotal roles in tropical and subtropical ecosystems. Thriving across diverse habitats, from rainforests to deserts, they harbor a multitude of mutualistic and antagonistic interactions with insects, nematodes, and pathogens. Despite their ecological significance, knowledge about the genomic background of Ficus remains limited. In this study, we report a chromosome-level reference genome of F. hirta, with a total size of 297.27 Mb, containing 28,625 protein-coding genes and 44.67% repeat sequences. These findings illuminate the genetic basis of Ficus responses to environmental challenges, offering valuable genomic resources for understanding genome size, adaptive evolution, and co-evolution with natural enemies and mutualists within the genus.2024-01-01T00:00:00Z2024 May2026-06-02T08:27:39.913Z2026-04-16T03:11:23.2ZS-EPMC111117943877806310.1038/s41597-024-03376-z