biostudies-literatureFang FMichigan State UniversityNational Cancer InstituteNCI NIH HHSAgBioResearch, Michigan State UniversityNational Institute of General Medical SciencesNIGMS NIH HHSNational Science Foundation109944https://www.ebi.ac.uk/biostudies/studies/S-EPMC11111832biostudies-literatureUnknown27(6)Maternal-to-zygotic transition (MZT) is central to early embryogenesis. However, its underlying molecular mechanisms are still not well described. Here, we revealed the expression dynamics of 5,000 proteins across four stages of zebrafish embryos during MZT, representing one of the most systematic surveys of proteome landscape of the zebrafish embryos during MZT. Nearly 700 proteins were differentially expressed and were divided into six clusters according to their expression patterns. The proteome expression profiles accurately reflect the main events that happen during the MZT, i.e., zygotic genome activation (ZGA), clearance of maternal mRNAs, and initiation of cellular differentiation and organogenesis. MZT is modulated by many proteins at multiple levels in a collaborative fashion, i.iScienceQuantitative proteomics reveals the dynamic proteome landscape of zebrafish embryos during the maternal-to-zygotic transition.PMC11111832R01GM118470DBI1846913R01 GM118470R01 CA247863R01CA247863R01GM125991R01 GM125991Liu XSun LWang QFang FCibelli JBBasharat ARChen DPoulos WfalseQuantitative proteomics reveals the dynamic proteome landscape of zebrafish embryos during the maternal-to-zygotic transition.Maternal-to-zygotic transition (MZT) is central to early embryogenesis. However, its underlying molecular mechanisms are still not well described. Here, we revealed the expression dynamics of 5,000 proteins across four stages of zebrafish embryos during MZT, representing one of the most systematic surveys of proteome landscape of the zebrafish embryos during MZT. Nearly 700 proteins were differentially expressed and were divided into six clusters according to their expression patterns. The proteome expression profiles accurately reflect the main events that happen during the MZT, i.e., zygotic genome activation (ZGA), clearance of maternal mRNAs, and initiation of cellular differentiation and organogenesis. MZT is modulated by many proteins at multiple levels in a collaborative fashion, i.2024-01-01T00:00:00Z2024 Jun2026-06-02T06:17:06.443Z2026-04-15T03:15:23.462ZS-EPMC111118323878401810.1016/j.isci.2024.109944