{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jacovetti C"],"funding":["University of Lausanne","Swiss National Science Foundation","University of Pisa","Ministero dell’Istruzione, dell’Università e della Ricerca","European Commission"],"pagination":["101955"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11112368"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84"],"pubmed_abstract":["<h4>Objective</h4>The contribution of the mitochondrial electron transfer system to insulin secretion involves more than just energy provision. We identified a small RNA fragment (mt-tRF-Leu<sup>TAA</sup>) derived from the cleavage of a mitochondrially-encoded tRNA that is conserved between mice and humans. The role of mitochondrially-encoded tRNA-derived fragments remains unknown. This study aimed to characterize the impact of mt-tRF-Leu<sup>TAA</sup>, on mitochondrial metabolism and pancreatic islet functions.<h4>Methods</h4>We used antisense oligonucleotides to reduce mt-tRF-Leu<sup>TAA</sup> levels in primary rat and human islet cells, as well as in insulin-secreting cell lines. We performed a joint transcriptome and proteome analysis upon mt-tRF-Leu<sup>TAA</sup> inhibition. Additiona"],"journal":["Molecular metabolism"],"pubmed_title":["The mitochondrial tRNA-derived fragment, mt-tRF-Leu<sup>TAA</sup>, couples mitochondrial metabolism to insulin secretion."],"pmcid":["PMC11112368"],"funding_grant_id":["PNRR M4C2-I1.3","194964","310030_219252","310030_188447","188447","310030","PE_00000019"],"pubmed_authors":["Jacovetti C","Wu K","Bouzakri K","Marchetti P","Sobel J","Regazzi R","Donnelly C","Menoud V","Cosentino C","Suleiman M","Kayser B","Guay C"],"additional_accession":[]},"is_claimable":false,"name":"The mitochondrial tRNA-derived fragment, mt-tRF-Leu<sup>TAA</sup>, couples mitochondrial metabolism to insulin secretion.","description":"<h4>Objective</h4>The contribution of the mitochondrial electron transfer system to insulin secretion involves more than just energy provision. We identified a small RNA fragment (mt-tRF-Leu<sup>TAA</sup>) derived from the cleavage of a mitochondrially-encoded tRNA that is conserved between mice and humans. The role of mitochondrially-encoded tRNA-derived fragments remains unknown. This study aimed to characterize the impact of mt-tRF-Leu<sup>TAA</sup>, on mitochondrial metabolism and pancreatic islet functions.<h4>Methods</h4>We used antisense oligonucleotides to reduce mt-tRF-Leu<sup>TAA</sup> levels in primary rat and human islet cells, as well as in insulin-secreting cell lines. We performed a joint transcriptome and proteome analysis upon mt-tRF-Leu<sup>TAA</sup> inhibition. Additiona","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-07-15T07:57:39.89Z","creation":"2026-07-01T03:12:11.1Z"},"accession":"S-EPMC11112368","cross_references":{"pubmed":["38704026"],"doi":["10.1016/j.molmet.2024.101955"]}}