biostudies-literatureUnknown70(19)Maixner FThe Tyrolean Iceman, a Copper-age ice mummy, is one of the best-studied human individuals. While the genome of the Iceman has largely been decoded, tissue-specific proteomes have not yet been investigated. We studied the proteome of two distinct brain samples using gel-based and liquid chromatography-mass spectrometry-based proteomics technologies together with a multiple-databases and -search algorithms-driven data-analysis approach. Thereby, we identified a total of 502 different proteins. Of these, 41 proteins are known to be highly abundant in brain tissue and 9 are even specifically expressed in the brain. Furthermore, we found 10 proteins related to blood and coagulation. An enrichment analysis revealed a significant accumulation of proteins related to stress response and wound healiCellular and molecular life sciences : CMLS3709-22https://www.ebi.ac.uk/biostudies/studies/S-EPMC11113858biostudies-literaturePaleoproteomic study of the Iceman's brain tissue.PMC11113858Schwarz AZink AMaixner FOverath TLinke DFranke AGuerriero Gvan den Berg BHLeidinger PTholey AMeder BKeller ABackes CEgarter-Vigl EMeese EJanko MKneissl BStade BJaremek MfalsePaleoproteomic study of the Iceman's brain tissue.The Tyrolean Iceman, a Copper-age ice mummy, is one of the best-studied human individuals. While the genome of the Iceman has largely been decoded, tissue-specific proteomes have not yet been investigated. We studied the proteome of two distinct brain samples using gel-based and liquid chromatography-mass spectrometry-based proteomics technologies together with a multiple-databases and -search algorithms-driven data-analysis approach. Thereby, we identified a total of 502 different proteins. Of these, 41 proteins are known to be highly abundant in brain tissue and 9 are even specifically expressed in the brain. Furthermore, we found 10 proteins related to blood and coagulation. An enrichment analysis revealed a significant accumulation of proteins related to stress response and wound heali2013-01-01T00:00:00Z2013 Oct2026-06-03T06:54:00.456Z2026-04-25T03:22:31.244ZS-EPMC111138582373994910.1007/s00018-013-1360-y