biostudies-literatureUnknown67(20)Cermak VMetastatic spreading of cancer cells is a highly complex process directed primarily by the interplay between tumor microenvironment, cell surface receptors, and actin cytoskeleton dynamics. To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines-the highly metastatic parental PR9692 and a non-metastasizing but fully tumorigenic clonal derivative PR9692-E9. Oligonucleotide microarray analysis of both cell lines revealed that the gene encoding the transcription factor EGR1 was downregulated in the non-metastatic PR9692-E9 cells. Further investigation demonstrated that the introduction of exogenous EGR1 into PR9692-E9 cells restored their metastatic potential to a level indistinguishablCellular and molecular life sciences : CMLS3557-68https://www.ebi.ac.uk/biostudies/studies/S-EPMC11115510biostudies-literatureThe transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells.PMC11115510Plachy JDvorak MTrejbalova KCermak VKosla JHejnar JfalseThe transcription factor EGR1 regulates metastatic potential of v-src transformed sarcoma cells.Metastatic spreading of cancer cells is a highly complex process directed primarily by the interplay between tumor microenvironment, cell surface receptors, and actin cytoskeleton dynamics. To advance our understanding of metastatic cancer dissemination, we have developed a model system that is based on two v-src transformed chicken sarcoma cell lines-the highly metastatic parental PR9692 and a non-metastasizing but fully tumorigenic clonal derivative PR9692-E9. Oligonucleotide microarray analysis of both cell lines revealed that the gene encoding the transcription factor EGR1 was downregulated in the non-metastatic PR9692-E9 cells. Further investigation demonstrated that the introduction of exogenous EGR1 into PR9692-E9 cells restored their metastatic potential to a level indistinguishabl2010-01-01T00:00:00Z2010 Oct2026-04-16T03:20:20.902Z2025-04-07T02:07:25.784ZS-EPMC111155102050597910.1007/s00018-010-0395-6