{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["67(9)"],"submitter":["Van den Abbeele A"],"pubmed_abstract":["RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its "],"journal":["Cellular and molecular life sciences : CMLS"],"pagination":["1519-35"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11115616"],"repository":["biostudies-literature"],"pubmed_title":["A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction."],"pmcid":["PMC11115616"],"pubmed_authors":["Steyaert J","De Corte V","Soror SH","De Clercq S","Van den Abbeele A","Vandekerckhove J","Gettemans J","De Ganck A","Van Loo B","Srinivasan V"],"additional_accession":[]},"is_claimable":false,"name":"A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.","description":"RNA interference has tremendously advanced our understanding of gene function but recent reports have exposed undesirable side-effects. Recombinant Camelid single-domain antibodies (VHHs) provide an attractive means for studying protein function without affecting gene expression. We raised VHHs against gelsolin (GsnVHHs), a multifunctional actin-binding protein that controls cellular actin organization and migration. GsnVHH-induced delocalization of gelsolin to mitochondria or the nucleus in mammalian cells reveals distinct subpopulations including free gelsolin and actin-bound gelsolin complexes. GsnVHH 13 specifically recognizes Ca(2+)-activated gelsolin (K (d) approximately 10 nM) while GsnVHH 11 binds gelsolin irrespective of Ca(2+) (K (d) approximately 5 nM) but completely blocks its ","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 May","modification":"2026-06-03T06:46:53.633Z","creation":"2026-05-29T03:05:57.02Z"},"accession":"S-EPMC11115616","cross_references":{"pubmed":["20140750"],"doi":["10.1007/s00018-010-0266-1"]}}