{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vanarsa K"],"funding":["NIH Clinical Center","NIAMS NIH HHS"],"pagination":["e000673"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11116855"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["<h4>Objective</h4>Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disorder with no reliable serum biomarkers currently available other than autoantibodies.<h4>Methods</h4>In the present study, isobaric tags for relative and absolute quantitation-based mass spectrometry was used to screen the sera of patients with SLE to uncover potential disease biomarkers.<h4>Results</h4>85 common proteins were identified, with 16 being elevated (≥1.3) and 23 being decreased (≤0.7) in SLE. Of the 16 elevated proteins, serum alpha-1-microglobulin/bikunin precursor (AMBP), zinc alpha-2 glycoprotein (AZGP) and retinol-binding protein 4 (RBP4) were validated in independent cross-sectional cohorts (Cohort I, N=52; Cohort II, N=117) using an orthogonal platform, ELISA. Serum AMBP, AZGP and R"],"journal":["Lupus science & medicine"],"pubmed_title":["iTRAQ-based mass spectrometry screen to identify serum biomarkers in systemic lupus erythematosus."],"pmcid":["PMC11116855"],"funding_grant_id":["R01 AR074096"],"pubmed_authors":["Hutcheson J","Nukala S","Vanarsa K","Zhang T","Mok CC","Saxena R","Kumar SR","Mohan C","Inthavong H","Wu T","Stanley B"],"additional_accession":[]},"is_claimable":false,"name":"iTRAQ-based mass spectrometry screen to identify serum biomarkers in systemic lupus erythematosus.","description":"<h4>Objective</h4>Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disorder with no reliable serum biomarkers currently available other than autoantibodies.<h4>Methods</h4>In the present study, isobaric tags for relative and absolute quantitation-based mass spectrometry was used to screen the sera of patients with SLE to uncover potential disease biomarkers.<h4>Results</h4>85 common proteins were identified, with 16 being elevated (≥1.3) and 23 being decreased (≤0.7) in SLE. Of the 16 elevated proteins, serum alpha-1-microglobulin/bikunin precursor (AMBP), zinc alpha-2 glycoprotein (AZGP) and retinol-binding protein 4 (RBP4) were validated in independent cross-sectional cohorts (Cohort I, N=52; Cohort II, N=117) using an orthogonal platform, ELISA. Serum AMBP, AZGP and R","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-04T01:48:55.236Z","creation":"2026-05-04T03:13:58.475Z"},"accession":"S-EPMC11116855","cross_references":{"pubmed":["38782493"],"doi":["10.1136/lupus-2022-000673"]}}