biostudies-literatureHe YNCI NIH HHSe2200905https://www.ebi.ac.uk/biostudies/studies/S-EPMC11117022biostudies-literatureUnknown11(15)With the advent of bioinformatic tools in efficiently predicting neo-antigens, peptide vaccines have gained tremendous attention in cancer immunotherapy. However, the delivery of peptide vaccines remains a major challenge, primarily due to ineffective transport to lymph nodes and low immunogenicity. Here, a strategy for peptide vaccine delivery is reported by first fusing the peptide to the cytosolic domain of the stimulator of interferon genes protein (STINGΔTM), then complexing the peptide-STINGΔTM protein with STING agonist 2'3' cyclic guanosine monophosphate-adenosine monophosphate (cGAMP). The process results in the formation of self-assembled cGAMP-peptide-STINGΔTM tetramers, which enables efficient lymphatic trafficking of the peptide. Moreover, the cGAMP-STINGΔTM complex acts not oAdvanced healthcare materialsPeptide-Based Cancer Vaccine Delivery via the STINGΔTM-cGAMP Complex.PMC11117022P30 CA014051Fletcher SJIrvine DJHuang SBelcher AMLi JHe YYang MBerger AGHammond PTSun XHong CfalsePeptide-Based Cancer Vaccine Delivery via the STINGΔTM-cGAMP Complex.With the advent of bioinformatic tools in efficiently predicting neo-antigens, peptide vaccines have gained tremendous attention in cancer immunotherapy. However, the delivery of peptide vaccines remains a major challenge, primarily due to ineffective transport to lymph nodes and low immunogenicity. Here, a strategy for peptide vaccine delivery is reported by first fusing the peptide to the cytosolic domain of the stimulator of interferon genes protein (STINGΔTM), then complexing the peptide-STINGΔTM protein with STING agonist 2'3' cyclic guanosine monophosphate-adenosine monophosphate (cGAMP). The process results in the formation of self-assembled cGAMP-peptide-STINGΔTM tetramers, which enables efficient lymphatic trafficking of the peptide. Moreover, the cGAMP-STINGΔTM complex acts not o2022-01-01T00:00:00Z2022 Aug2026-07-15T07:55:39.294Z2026-07-01T03:11:02.236ZS-EPMC111170223567024410.1002/adhm.202200905