biostudies-literatureFrantsuzova ERussian Science Foundation339https://www.ebi.ac.uk/biostudies/studies/S-EPMC11117675biostudies-literatureUnknown13(5)The strain <i>Gordonia polyisoprenivorans</i> 135 is able to utilize a wide range of aromatic compounds. The aim of this work was to study the features of genetic organization and biotechnological potential of the strain <i>G. polyisoprenivorans</i> 135 as a degrader of aromatic compounds. The study of the genome of the strain 135 and the pangenome of the <i>G. polyisoprenivorans</i> species revealed that some genes, presumably involved in PAH catabolism, are atypical for <i>Gordonia</i> and belong to the pangenome of <i>Actinobacteria</i>. Analyzing the intergenic regions of strain 135 alongside the "panIGRome" of <i>G. polyisoprenivorans</i> showed that some intergenic regions in strain 135 also differ from those located between the same pairs of genes in related strains. The strain <i>G. polyisoprenivorans</i> 135 in our work utilized naphthalene (degradation degree 39.43%) and grew actively on salicylate. At present, this is the only known strain of <i>G. polyisoprenivorans</i> with experimentally confirmed ability to utilize these compounds.BiologyGenomic, Phylogenetic and Physiological Characterization of the PAH-Degrading Strain <i>Gordonia polyisoprenivorans</i> 135.PMC1111767522-74-10082Solyanikova IBogun ADelegan YStreletskii RFrantsuzova EVetrova AKopylova OfalseGenomic, Phylogenetic and Physiological Characterization of the PAH-Degrading Strain <i>Gordonia polyisoprenivorans</i> 135.The strain <i>Gordonia polyisoprenivorans</i> 135 is able to utilize a wide range of aromatic compounds. The aim of this work was to study the features of genetic organization and biotechnological potential of the strain <i>G. polyisoprenivorans</i> 135 as a degrader of aromatic compounds. The study of the genome of the strain 135 and the pangenome of the <i>G. polyisoprenivorans</i> species revealed that some genes, presumably involved in PAH catabolism, are atypical for <i>Gordonia</i> and belong to the pangenome of <i>Actinobacteria</i>. Analyzing the intergenic regions of strain 135 alongside the "panIGRome" of <i>G. polyisoprenivorans</i> showed that some intergenic regions in strain 135 also differ from those located between the same pairs of genes in related strains. The strain <i>G. polyisoprenivorans</i> 135 in our work utilized naphthalene (degradation degree 39.43%) and grew actively on salicylate. At present, this is the only known strain of <i>G. polyisoprenivorans</i> with experimentally confirmed ability to utilize these compounds.2024-01-01T00:00:00Z2024 May2026-04-19T03:28:40.644Z2026-04-19T03:14:07.843ZS-EPMC111176753878582110.3390/biology13050339