biostudies-literatureUnknown13(5)Elzhraa FThe spread of superbugs in dairy products can jeopardize global public health. To date, information on the incidence rates of virulent and β-lactams-resistant (BLR) <i>Salmonella</i> in cheeses from rural areas of Egypt has been lacking. Biochemical, serological, antibiotic susceptibility, and multiplex PCR (M-PCR) tests were performed to identify and characterize <i>Salmonella</i> isolates. In this study, 44 (15.71%) <i>Salmonella</i> isolates of eight different serotypes were recovered from 280 samples of Rumi and pasteurized Kariesh cheeses across the Nile Delta region of Egypt. The most predominant serotypes were <i>S</i>. Typhimurium, <i>S</i>. Enteritidis, and <i>S</i>. Infantis. The virulence genes (<i>invA</i>, <i>stn</i>, and <i>hilA</i>) were identified in all isolates. However, Antibiotics (Basel, Switzerland)454https://www.ebi.ac.uk/biostudies/studies/S-EPMC11117923biostudies-literatureRumi and Pasteurized Kareish Cheeses Are a Source of β-Lactam-Resistant <i>Salmonella</i> in the Nile Delta Region of Egypt: Insights into Their Incidence, AMR Pattern, Genotypic Determinants of Virulence and β-Lactam Resistance.PMC11117923Belak AEl-Sherbini MElzhraa FKisko GMohacsi-Farkas CAl-Ashmawy MEl-Sebaey AMfalseRumi and Pasteurized Kareish Cheeses Are a Source of β-Lactam-Resistant <i>Salmonella</i> in the Nile Delta Region of Egypt: Insights into Their Incidence, AMR Pattern, Genotypic Determinants of Virulence and β-Lactam Resistance.The spread of superbugs in dairy products can jeopardize global public health. To date, information on the incidence rates of virulent and β-lactams-resistant (BLR) <i>Salmonella</i> in cheeses from rural areas of Egypt has been lacking. Biochemical, serological, antibiotic susceptibility, and multiplex PCR (M-PCR) tests were performed to identify and characterize <i>Salmonella</i> isolates. In this study, 44 (15.71%) <i>Salmonella</i> isolates of eight different serotypes were recovered from 280 samples of Rumi and pasteurized Kariesh cheeses across the Nile Delta region of Egypt. The most predominant serotypes were <i>S</i>. Typhimurium, <i>S</i>. Enteritidis, and <i>S</i>. Infantis. The virulence genes (<i>invA</i>, <i>stn</i>, and <i>hilA</i>) were identified in all isolates. However, 2024-01-01T00:00:00Z2024 May2025-08-31T03:11:17.325Z2025-08-31T03:05:16.994ZS-EPMC111179233878618510.3390/antibiotics13050454