{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramatla T"],"funding":["National Research Foundation"],"pagination":["458"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11117945"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(5)"],"pubmed_abstract":["Rapid growth in commercial poultry production is one of the major sources of <i>Salmonella</i> infections that leads to human salmonellosis. The two main <i>Salmonella enterica</i> serovars associated with human salmonellosis are enteritidis and typhimurium. The aim of this study was to determine the prevalence of <i>S. enterica</i> serovars Enteritidis and <i>S.</i> Typhimurium as well as their <i>Salmonella</i> pathogenicity islands (SPI) and antibiotic resistance profiles in broiler chicken feces from slaughterhouses. A total of 480 fecal samples from broiler chickens that were grouped into 96 pooled samples were identified to have <i>Salmonella</i> spp. using the <i>invA</i> gene, whilst the <i>Spy</i> and <i>sdfI</i> genes were used to screen for the presence of <i>S.</i> Enteritidis and <i>S.</i> Typhimurium serovars, respectively, by polymerase chain reaction (PCR) assays. The isolates were also screened for the presence of <i>Salmonella</i> pathogenicity islands (SPIs) using PCR. The disc diffusion assay was performed to determine the antibiotic resistance profiles of the isolates. A total of 36 isolates were confirmed as <i>Salmonella</i> spp. through amplification of the <i>invA</i> gene. Out of 36 confirmed <i>Salmonella</i> spp. a total of 22 isolates were classified as <i>S</i>. Enteritidis (n = 8) and were <i>S.</i> Typhimurium (n = 14) serovars. All (n = 22) <i>S.</i> Enteritidis and <i>S.</i> Typhimurium isolates possessed the <i>hilA</i> (SPI-1), <i>ssrB</i> (SPI-2) and <i>pagC</i> (SPI-11) pathogenicity islands genes. Amongst these serovars, 50% of the isolates (n = 11/22) were resistant to tetracycline and nalidixic acid. Only 22% of the isolates, <i>S.</i> Typhimurium (13.6%) and <i>S.</i> Enteritidis (9.1%) demonstrated resistance against three or more antibiotic classes. The most detected antibiotic resistance genes were <i>tet</i>(<i>K</i>), <i>mcr-1</i>, <i>sulI</i> and <i>strA</i> with 13 (59.1%), 9 (40.9%), 9 (40.9%) and 7 (31.8%), respectively. The findings of this study revealed that <i>S.</i> Typhimurium is the most prevalent serotype detected in chicken feces. To reduce the risk to human health posed by salmonellosis, a stringent public health and food safety policy is required."],"journal":["Antibiotics (Basel, Switzerland)"],"pubmed_title":["Detection of <i>Salmonella</i> Pathogenicity Islands and Antimicrobial-Resistant Genes in <i>Salmonella enterica</i> Serovars Enteritidis and Typhimurium Isolated from Broiler Chickens."],"pmcid":["PMC11117945"],"funding_grant_id":["118949"],"pubmed_authors":["Mokgokong P","Ndou R","Ramaili T","Lekota K","Mlangeni LN","Thekisoe O","Nkhebenyane JS","Ramatla T","Khasapane NG"],"additional_accession":[]},"is_claimable":false,"name":"Detection of <i>Salmonella</i> Pathogenicity Islands and Antimicrobial-Resistant Genes in <i>Salmonella enterica</i> Serovars Enteritidis and Typhimurium Isolated from Broiler Chickens.","description":"Rapid growth in commercial poultry production is one of the major sources of <i>Salmonella</i> infections that leads to human salmonellosis. The two main <i>Salmonella enterica</i> serovars associated with human salmonellosis are enteritidis and typhimurium. The aim of this study was to determine the prevalence of <i>S. enterica</i> serovars Enteritidis and <i>S.</i> Typhimurium as well as their <i>Salmonella</i> pathogenicity islands (SPI) and antibiotic resistance profiles in broiler chicken feces from slaughterhouses. A total of 480 fecal samples from broiler chickens that were grouped into 96 pooled samples were identified to have <i>Salmonella</i> spp. using the <i>invA</i> gene, whilst the <i>Spy</i> and <i>sdfI</i> genes were used to screen for the presence of <i>S.</i> Enteritidis and <i>S.</i> Typhimurium serovars, respectively, by polymerase chain reaction (PCR) assays. The isolates were also screened for the presence of <i>Salmonella</i> pathogenicity islands (SPIs) using PCR. The disc diffusion assay was performed to determine the antibiotic resistance profiles of the isolates. A total of 36 isolates were confirmed as <i>Salmonella</i> spp. through amplification of the <i>invA</i> gene. Out of 36 confirmed <i>Salmonella</i> spp. a total of 22 isolates were classified as <i>S</i>. Enteritidis (n = 8) and were <i>S.</i> Typhimurium (n = 14) serovars. All (n = 22) <i>S.</i> Enteritidis and <i>S.</i> Typhimurium isolates possessed the <i>hilA</i> (SPI-1), <i>ssrB</i> (SPI-2) and <i>pagC</i> (SPI-11) pathogenicity islands genes. Amongst these serovars, 50% of the isolates (n = 11/22) were resistant to tetracycline and nalidixic acid. Only 22% of the isolates, <i>S.</i> Typhimurium (13.6%) and <i>S.</i> Enteritidis (9.1%) demonstrated resistance against three or more antibiotic classes. The most detected antibiotic resistance genes were <i>tet</i>(<i>K</i>), <i>mcr-1</i>, <i>sulI</i> and <i>strA</i> with 13 (59.1%), 9 (40.9%), 9 (40.9%) and 7 (31.8%), respectively. The findings of this study revealed that <i>S.</i> Typhimurium is the most prevalent serotype detected in chicken feces. To reduce the risk to human health posed by salmonellosis, a stringent public health and food safety policy is required.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-04-16T03:22:22.889Z","creation":"2026-04-16T03:13:02.125Z"},"accession":"S-EPMC11117945","cross_references":{"pubmed":["38786186"],"doi":["10.3390/antibiotics13050458"]}}