biostudies-literatureUnknownLi OQNCATS NIH HHSNIDDK NIH HHSNHLBI NIH HHSNIGMS NIH HHS<h4>Background</h4>Thrombosis is a major cause of myocardial infarction and ischemic stroke. The sodium/potassium ATPase (NKA), comprising α and β subunits, is crucial in maintaining intracellular sodium and potassium gradients. However, the role of NKA in platelet function and thrombosis remains unclear.<h4>Methods</h4>We utilized wild-type (WT, α<i>1</i> ^+/+) and NKA α1 heterozygous (α<i>1</i> ^+/-) mice, aged 8 to 16 weeks, of both sexes. An intravital microscopy-based, FeCl₃-induced carotid artery injury thrombosis model was employed for in vivo thrombosis assessment. Platelet transfusion assays were used to evaluate platelet NKA α1 function on thrombosis. Human platelets isolated from healthy donors and heart failure patients treated with/without digoxibioRxiv : the preprint server for biology2024.05.13.593923https://www.ebi.ac.uk/biostudies/studies/S-EPMC11118499biostudies-literatureSodium/Potassium ATPase Alpha 1 Subunit Fine-tunes Platelet GPCR Signaling Function and is Essential for Thrombosis.PMC11118499R15 HL145666UL1 TR003017R15 HL164682P20 GM103434R01 DK129937R15 HL145573U54 GM104942Kennedy DLiu JRoytenberg RAguilar RYue HOlanipekun OThompson ELi WDeHart ARFedorova OLi OQPierre SVBai FfalseSodium/Potassium ATPase Alpha 1 Subunit Fine-tunes Platelet GPCR Signaling Function and is Essential for Thrombosis.<h4>Background</h4>Thrombosis is a major cause of myocardial infarction and ischemic stroke. The sodium/potassium ATPase (NKA), comprising α and β subunits, is crucial in maintaining intracellular sodium and potassium gradients. However, the role of NKA in platelet function and thrombosis remains unclear.<h4>Methods</h4>We utilized wild-type (WT, α<i>1</i> ^+/+) and NKA α1 heterozygous (α<i>1</i> ^+/-) mice, aged 8 to 16 weeks, of both sexes. An intravital microscopy-based, FeCl₃-induced carotid artery injury thrombosis model was employed for in vivo thrombosis assessment. Platelet transfusion assays were used to evaluate platelet NKA α1 function on thrombosis. Human platelets isolated from healthy donors and heart failure patients treated with/without digoxi2024-01-01T00:00:00Z2024 May2026-06-26T03:18:17.322Z2026-06-26T03:09:28.807ZS-EPMC111184993879855610.1101/2024.05.13.593923