{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Noll JCG"],"funding":["Cornell Atkinson Center for Sustainability","National Pork Board","National Institute of Food and Agriculture","USDA National Institute of Food and Agriculture","Cornell Atkinson Center for Sustainability—Graduate Research Grant in Food Security"],"pagination":["758"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11125664"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(5)"],"pubmed_abstract":["African Swine Fever Virus (ASFV) is a large dsDNA virus that encodes at least 150 proteins. The complexity of ASFV and lack of knowledge of effector immune functions and protective antigens have hindered the development of safe and effective ASF vaccines. In this study, we constructed four Orf virus recombinant vectors expressing individual ASFV genes <i>B602L</i>, -<i>CP204L</i>, <i>E184L</i>, and -<i>I73R</i> (ORFV<sup>Δ121</sup>-ASFV-<i>B602L</i>, -<i>CP204L</i>, -<i>E184L</i>, and -<i>I73R</i>). All recombinant viruses expressed the heterologous ASFV proteins in vitro. We then evaluated the immunogenicity of the recombinants by immunizing four-week-old piglets. In two independent animal studies, we observed high antibody titers against ASFV p30, encoded by <i>CP204L</i> gene. Using Pep"],"journal":["Viruses"],"pubmed_title":["Identification of an Immunodominant B-Cell Epitope in African Swine Fever Virus p30 Protein and Evidence of p30 Antibody-Mediated Antibody Dependent Cellular Cytotoxicity."],"pmcid":["PMC11125664"],"funding_grant_id":["Graduate Research Grant in Food Security","NPB#22-081","NYCV478910"],"pubmed_authors":["do Nascimento GM","Caserta LC","Diel DG","Noll JCG","Fernandes MHV","Rani R","Butt SL","Covaleda L","Martins M"],"additional_accession":[]},"is_claimable":false,"name":"Identification of an Immunodominant B-Cell Epitope in African Swine Fever Virus p30 Protein and Evidence of p30 Antibody-Mediated Antibody Dependent Cellular Cytotoxicity.","description":"African Swine Fever Virus (ASFV) is a large dsDNA virus that encodes at least 150 proteins. The complexity of ASFV and lack of knowledge of effector immune functions and protective antigens have hindered the development of safe and effective ASF vaccines. In this study, we constructed four Orf virus recombinant vectors expressing individual ASFV genes <i>B602L</i>, -<i>CP204L</i>, <i>E184L</i>, and -<i>I73R</i> (ORFV<sup>Δ121</sup>-ASFV-<i>B602L</i>, -<i>CP204L</i>, -<i>E184L</i>, and -<i>I73R</i>). All recombinant viruses expressed the heterologous ASFV proteins in vitro. We then evaluated the immunogenicity of the recombinants by immunizing four-week-old piglets. In two independent animal studies, we observed high antibody titers against ASFV p30, encoded by <i>CP204L</i> gene. Using Pep","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-04-24T03:19:44.713Z","creation":"2026-04-24T03:09:53.469Z"},"accession":"S-EPMC11125664","cross_references":{"pubmed":["38793639"],"doi":["10.3390/v16050758"]}}