biostudies-literatureGully KDeutsche Forschungsgemeinschaft (DFG)Swiss National Science FoundationDeutsche Forschungsgemeinschafte2314570121https://www.ebi.ac.uk/biostudies/studies/S-EPMC11127019biostudies-literatureUnknown121(21)Lipid polymers such as cutin and suberin strengthen the diffusion barrier properties of the cell wall in specific cell types and are essential for water relations, mineral nutrition, and stress protection in plants. Land plant-specific glycerol-3-phosphate acyltransferases (GPATs) of different clades are central players in cutin and suberin monomer biosynthesis. Here, we show that the <i>GPAT4</i>/<i>6</i>/<i>8</i> clade in <i>Arabidopsis thaliana</i>, which is known to mediate cutin formation, is also required for developmentally regulated root suberization, in addition to the established roles of <i>GPAT5/7</i> in suberization. The <i>GPAT5</i>/<i>7</i> clade is mainly required for abscisic acid-regulated suberization. In addition, the <i>GPAT5</i>/<i>7</i> clade is crucial for the formaProceedings of the National Academy of Sciences of the United States of AmericaThe <i>GPAT4</i>/<i>6</i>/<i>8</i> clade functions in Arabidopsis root suberization nonredundantly with the <i>GPAT5/7</i> clade required for suberin lamellae.PMC1112701931003A_170127170127INST 186/1167-1310030188672310030_188672Gully KHerrfurth CDe Bellis DBerhin AFeussner INawrath CfalseThe <i>GPAT4</i>/<i>6</i>/<i>8</i> clade functions in Arabidopsis root suberization nonredundantly with the <i>GPAT5/7</i> clade required for suberin lamellae.Lipid polymers such as cutin and suberin strengthen the diffusion barrier properties of the cell wall in specific cell types and are essential for water relations, mineral nutrition, and stress protection in plants. Land plant-specific glycerol-3-phosphate acyltransferases (GPATs) of different clades are central players in cutin and suberin monomer biosynthesis. Here, we show that the <i>GPAT4</i>/<i>6</i>/<i>8</i> clade in <i>Arabidopsis thaliana</i>, which is known to mediate cutin formation, is also required for developmentally regulated root suberization, in addition to the established roles of <i>GPAT5/7</i> in suberization. The <i>GPAT5</i>/<i>7</i> clade is mainly required for abscisic acid-regulated suberization. In addition, the <i>GPAT5</i>/<i>7</i> clade is crucial for the forma2024-01-01T00:00:00Z2024 May2026-07-09T12:12:54.514Z2025-04-04T00:10:47.939ZS-EPMC111270193873980410.1073/pnas.2314570121